Enhanced top-down characterization of histone post-translational modifications

Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tand...

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Detalles Bibliográficos
Autores principales: Tian, Zhixin, Tolić, Nikola, Zhao, Rui, Moore, Ronald J, Hengel, Shawna M, Robinson, Errol W, Stenoien, David L, Wu, Si, Smith, Richard D, Paša-Tolić, Ljiljana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3491414/
https://www.ncbi.nlm.nih.gov/pubmed/23034525
http://dx.doi.org/10.1186/gb-2012-13-10-r86
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author Tian, Zhixin
Tolić, Nikola
Zhao, Rui
Moore, Ronald J
Hengel, Shawna M
Robinson, Errol W
Stenoien, David L
Wu, Si
Smith, Richard D
Paša-Tolić, Ljiljana
author_facet Tian, Zhixin
Tolić, Nikola
Zhao, Rui
Moore, Ronald J
Hengel, Shawna M
Robinson, Errol W
Stenoien, David L
Wu, Si
Smith, Richard D
Paša-Tolić, Ljiljana
author_sort Tian, Zhixin
collection PubMed
description Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 µg purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms.
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spelling pubmed-34914142012-11-08 Enhanced top-down characterization of histone post-translational modifications Tian, Zhixin Tolić, Nikola Zhao, Rui Moore, Ronald J Hengel, Shawna M Robinson, Errol W Stenoien, David L Wu, Si Smith, Richard D Paša-Tolić, Ljiljana Genome Biol Method Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 µg purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms. BioMed Central 2012 2012-10-03 /pmc/articles/PMC3491414/ /pubmed/23034525 http://dx.doi.org/10.1186/gb-2012-13-10-r86 Text en Copyright ©2012 Tian et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Tian, Zhixin
Tolić, Nikola
Zhao, Rui
Moore, Ronald J
Hengel, Shawna M
Robinson, Errol W
Stenoien, David L
Wu, Si
Smith, Richard D
Paša-Tolić, Ljiljana
Enhanced top-down characterization of histone post-translational modifications
title Enhanced top-down characterization of histone post-translational modifications
title_full Enhanced top-down characterization of histone post-translational modifications
title_fullStr Enhanced top-down characterization of histone post-translational modifications
title_full_unstemmed Enhanced top-down characterization of histone post-translational modifications
title_short Enhanced top-down characterization of histone post-translational modifications
title_sort enhanced top-down characterization of histone post-translational modifications
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3491414/
https://www.ncbi.nlm.nih.gov/pubmed/23034525
http://dx.doi.org/10.1186/gb-2012-13-10-r86
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