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A reproducible, high throughput method for fabricating fibrin gels

BACKGROUND: Fibrin gels are a promising biomaterial for tissue engineering. However, current fabrication methods are time intensive with inherent variation. There is a pressing need to develop new and consistent approaches for producing fibrin-based hydrogels for examination. FINDINGS: We developed...

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Detalles Bibliográficos
Autores principales: Murphy, Kaitlin C, Leach, J Kent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492004/
https://www.ncbi.nlm.nih.gov/pubmed/22873708
http://dx.doi.org/10.1186/1756-0500-5-423
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author Murphy, Kaitlin C
Leach, J Kent
author_facet Murphy, Kaitlin C
Leach, J Kent
author_sort Murphy, Kaitlin C
collection PubMed
description BACKGROUND: Fibrin gels are a promising biomaterial for tissue engineering. However, current fabrication methods are time intensive with inherent variation. There is a pressing need to develop new and consistent approaches for producing fibrin-based hydrogels for examination. FINDINGS: We developed a high throughput method for creating fibrin gels using molds fabricated from polydimethylsiloxane (PDMS). Fibrin gels were produced by adding solutions of fibrinogen and thrombin to cylindrical defects in a PDMS sheet. Undisturbed gels were collected by removing the sheet, and fibrin gels were characterized. The characteristics of resulting gels were compared to published data by measuring compressive stiffness and osteogenic response of entrapped human mesenchymal stem cells (MSCs). Gels exhibited compressive moduli nearly identical to our previously reported fabrication method. Trends in alkaline phosphatase activity, an early marker of osteogenic differentiation in MSCs, were also consistent with previous data. CONCLUSIONS: These findings demonstrate a streamlined approach to fibrin gel production that drastically reduces the time required to make fibrin gels, while also reducing variability between gel batches. This fabrication technique provides a valuable tool for generating large numbers of gels in a cost-effective manner.
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spelling pubmed-34920042012-11-08 A reproducible, high throughput method for fabricating fibrin gels Murphy, Kaitlin C Leach, J Kent BMC Res Notes Technical Note BACKGROUND: Fibrin gels are a promising biomaterial for tissue engineering. However, current fabrication methods are time intensive with inherent variation. There is a pressing need to develop new and consistent approaches for producing fibrin-based hydrogels for examination. FINDINGS: We developed a high throughput method for creating fibrin gels using molds fabricated from polydimethylsiloxane (PDMS). Fibrin gels were produced by adding solutions of fibrinogen and thrombin to cylindrical defects in a PDMS sheet. Undisturbed gels were collected by removing the sheet, and fibrin gels were characterized. The characteristics of resulting gels were compared to published data by measuring compressive stiffness and osteogenic response of entrapped human mesenchymal stem cells (MSCs). Gels exhibited compressive moduli nearly identical to our previously reported fabrication method. Trends in alkaline phosphatase activity, an early marker of osteogenic differentiation in MSCs, were also consistent with previous data. CONCLUSIONS: These findings demonstrate a streamlined approach to fibrin gel production that drastically reduces the time required to make fibrin gels, while also reducing variability between gel batches. This fabrication technique provides a valuable tool for generating large numbers of gels in a cost-effective manner. BioMed Central 2012-08-08 /pmc/articles/PMC3492004/ /pubmed/22873708 http://dx.doi.org/10.1186/1756-0500-5-423 Text en Copyright ©2012 Murphy and Leach; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
Murphy, Kaitlin C
Leach, J Kent
A reproducible, high throughput method for fabricating fibrin gels
title A reproducible, high throughput method for fabricating fibrin gels
title_full A reproducible, high throughput method for fabricating fibrin gels
title_fullStr A reproducible, high throughput method for fabricating fibrin gels
title_full_unstemmed A reproducible, high throughput method for fabricating fibrin gels
title_short A reproducible, high throughput method for fabricating fibrin gels
title_sort reproducible, high throughput method for fabricating fibrin gels
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492004/
https://www.ncbi.nlm.nih.gov/pubmed/22873708
http://dx.doi.org/10.1186/1756-0500-5-423
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