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RhoC Regulates the Proliferation of Gastric Cancer Cells through Interaction with IQGAP1

Our previous research results showed that both Ras homolog family member C (RhoC) and IQ-domain GTPase-activating protein 1 (IQGAP1) were over-expressed in gastric cancer tissues and cells, but their role in tumorigenensis has not been addressed clearly. Herein we reported the proliferation stimulat...

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Autores principales: Wu, Yan, Tao, Yan, Chen, Yongchang, Xu, Wenrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492142/
https://www.ncbi.nlm.nih.gov/pubmed/23145020
http://dx.doi.org/10.1371/journal.pone.0048917
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author Wu, Yan
Tao, Yan
Chen, Yongchang
Xu, Wenrong
author_facet Wu, Yan
Tao, Yan
Chen, Yongchang
Xu, Wenrong
author_sort Wu, Yan
collection PubMed
description Our previous research results showed that both Ras homolog family member C (RhoC) and IQ-domain GTPase-activating protein 1 (IQGAP1) were over-expressed in gastric cancer tissues and cells, but their role in tumorigenensis has not been addressed clearly. Herein we reported the proliferation stimulating effect of RhoC and IQGAP1 on gastric cancer cells and the interaction between two proteins in regulating the proliferation of gastric cancer cells. Plasmids and viral constructs encoding target siRNA and DNA were used to alter the expression of RhoC and IQGAP1. MTT method and BrdU incorporation assay were used for analyzing the effect of RhoC and different structures of IQGAP1 on proliferation. Protein levels of IQGAP1 and RhoC in cell lines were detected by Western blotting. Immunofluorescence and Co-Immunoprecipitation assays were applied to investigate the localization and binding between RhoC and IQGAP1. The results showed that RhoC, IQGAP1 and the C-terminal fragment of IQGAP1 significantly stimulated the proliferation of gastric cancer cells, and enhanced the expression of cyclin E and cyclin D1. By contrast, reduction of endogenous IQGAP1 or RhoC by siRNA attenuated cell proliferation. The depletion of IQGAP1 expression by siRNA significantly blocked the proliferative activity of constitutively active RhoC, while RhoC silencing by siRNA had no effect on IQGAP1-induced proliferation in gastric cancer cells. Co-immunoprecipitation and Immunofluorescence assays showed that RhoC and IQGAP1 bound each other. In conclusion, our results suggest that RhoC stimulates the proliferation of gastric cancer cells through recruiting IQGAP1 as an effector.
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spelling pubmed-34921422012-11-09 RhoC Regulates the Proliferation of Gastric Cancer Cells through Interaction with IQGAP1 Wu, Yan Tao, Yan Chen, Yongchang Xu, Wenrong PLoS One Research Article Our previous research results showed that both Ras homolog family member C (RhoC) and IQ-domain GTPase-activating protein 1 (IQGAP1) were over-expressed in gastric cancer tissues and cells, but their role in tumorigenensis has not been addressed clearly. Herein we reported the proliferation stimulating effect of RhoC and IQGAP1 on gastric cancer cells and the interaction between two proteins in regulating the proliferation of gastric cancer cells. Plasmids and viral constructs encoding target siRNA and DNA were used to alter the expression of RhoC and IQGAP1. MTT method and BrdU incorporation assay were used for analyzing the effect of RhoC and different structures of IQGAP1 on proliferation. Protein levels of IQGAP1 and RhoC in cell lines were detected by Western blotting. Immunofluorescence and Co-Immunoprecipitation assays were applied to investigate the localization and binding between RhoC and IQGAP1. The results showed that RhoC, IQGAP1 and the C-terminal fragment of IQGAP1 significantly stimulated the proliferation of gastric cancer cells, and enhanced the expression of cyclin E and cyclin D1. By contrast, reduction of endogenous IQGAP1 or RhoC by siRNA attenuated cell proliferation. The depletion of IQGAP1 expression by siRNA significantly blocked the proliferative activity of constitutively active RhoC, while RhoC silencing by siRNA had no effect on IQGAP1-induced proliferation in gastric cancer cells. Co-immunoprecipitation and Immunofluorescence assays showed that RhoC and IQGAP1 bound each other. In conclusion, our results suggest that RhoC stimulates the proliferation of gastric cancer cells through recruiting IQGAP1 as an effector. Public Library of Science 2012-11-07 /pmc/articles/PMC3492142/ /pubmed/23145020 http://dx.doi.org/10.1371/journal.pone.0048917 Text en © 2012 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wu, Yan
Tao, Yan
Chen, Yongchang
Xu, Wenrong
RhoC Regulates the Proliferation of Gastric Cancer Cells through Interaction with IQGAP1
title RhoC Regulates the Proliferation of Gastric Cancer Cells through Interaction with IQGAP1
title_full RhoC Regulates the Proliferation of Gastric Cancer Cells through Interaction with IQGAP1
title_fullStr RhoC Regulates the Proliferation of Gastric Cancer Cells through Interaction with IQGAP1
title_full_unstemmed RhoC Regulates the Proliferation of Gastric Cancer Cells through Interaction with IQGAP1
title_short RhoC Regulates the Proliferation of Gastric Cancer Cells through Interaction with IQGAP1
title_sort rhoc regulates the proliferation of gastric cancer cells through interaction with iqgap1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492142/
https://www.ncbi.nlm.nih.gov/pubmed/23145020
http://dx.doi.org/10.1371/journal.pone.0048917
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