Cargando…

Metabolic Engineering Plant Seeds with Fish Oil-Like Levels of DHA

BACKGROUND: Omega-3 long-chain (≥C(20)) polyunsaturated fatty acids (ω3 LC-PUFA) have critical roles in human health and development with studies indicating that deficiencies in these fatty acids can increase the risk or severity of cardiovascular and inflammatory diseases in particular. These fatty...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrie, James R., Shrestha, Pushkar, Zhou, Xue-Rong, Mansour, Maged P., Liu, Qing, Belide, Srinivas, Nichols, Peter D., Singh, Surinder P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492320/
https://www.ncbi.nlm.nih.gov/pubmed/23145108
http://dx.doi.org/10.1371/journal.pone.0049165
_version_ 1782249108489109504
author Petrie, James R.
Shrestha, Pushkar
Zhou, Xue-Rong
Mansour, Maged P.
Liu, Qing
Belide, Srinivas
Nichols, Peter D.
Singh, Surinder P.
author_facet Petrie, James R.
Shrestha, Pushkar
Zhou, Xue-Rong
Mansour, Maged P.
Liu, Qing
Belide, Srinivas
Nichols, Peter D.
Singh, Surinder P.
author_sort Petrie, James R.
collection PubMed
description BACKGROUND: Omega-3 long-chain (≥C(20)) polyunsaturated fatty acids (ω3 LC-PUFA) have critical roles in human health and development with studies indicating that deficiencies in these fatty acids can increase the risk or severity of cardiovascular and inflammatory diseases in particular. These fatty acids are predominantly sourced from fish and algal oils, but it is widely recognised that there is an urgent need for an alternative and sustainable source of EPA and DHA. Since the earliest demonstrations of ω3 LC-PUFA engineering there has been good progress in engineering the C(20) EPA with seed fatty acid levels similar to that observed in bulk fish oil (∼18%), although undesirable ω6 PUFA levels have also remained high. METHODOLOGY/PRINCIPAL FINDINGS: The transgenic seed production of the particularly important C(22) DHA has been problematic with many attempts resulting in the accumulation of EPA/DPA, but only a few percent of DHA. This study describes the production of up to 15% of the C(22) fatty acid DHA in Arabidopsis thaliana seed oil with a high ω3/ω6 ratio. This was achieved using a transgenic pathway to increase the C(18) ALA which was then converted to DHA by a microalgal Δ6-desaturase pathway. CONCLUSIONS/SIGNIFICANCE: The amount of DHA described in this study exceeds the 12% level at which DHA is generally found in bulk fish oil. This is a breakthrough in the development of sustainable alternative sources of DHA as this technology should be applicable in oilseed crops. One hectare of a Brassica napus crop containing 12% DHA in seed oil would produce as much DHA as approximately 10,000 fish.
format Online
Article
Text
id pubmed-3492320
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34923202012-11-09 Metabolic Engineering Plant Seeds with Fish Oil-Like Levels of DHA Petrie, James R. Shrestha, Pushkar Zhou, Xue-Rong Mansour, Maged P. Liu, Qing Belide, Srinivas Nichols, Peter D. Singh, Surinder P. PLoS One Research Article BACKGROUND: Omega-3 long-chain (≥C(20)) polyunsaturated fatty acids (ω3 LC-PUFA) have critical roles in human health and development with studies indicating that deficiencies in these fatty acids can increase the risk or severity of cardiovascular and inflammatory diseases in particular. These fatty acids are predominantly sourced from fish and algal oils, but it is widely recognised that there is an urgent need for an alternative and sustainable source of EPA and DHA. Since the earliest demonstrations of ω3 LC-PUFA engineering there has been good progress in engineering the C(20) EPA with seed fatty acid levels similar to that observed in bulk fish oil (∼18%), although undesirable ω6 PUFA levels have also remained high. METHODOLOGY/PRINCIPAL FINDINGS: The transgenic seed production of the particularly important C(22) DHA has been problematic with many attempts resulting in the accumulation of EPA/DPA, but only a few percent of DHA. This study describes the production of up to 15% of the C(22) fatty acid DHA in Arabidopsis thaliana seed oil with a high ω3/ω6 ratio. This was achieved using a transgenic pathway to increase the C(18) ALA which was then converted to DHA by a microalgal Δ6-desaturase pathway. CONCLUSIONS/SIGNIFICANCE: The amount of DHA described in this study exceeds the 12% level at which DHA is generally found in bulk fish oil. This is a breakthrough in the development of sustainable alternative sources of DHA as this technology should be applicable in oilseed crops. One hectare of a Brassica napus crop containing 12% DHA in seed oil would produce as much DHA as approximately 10,000 fish. Public Library of Science 2012-11-07 /pmc/articles/PMC3492320/ /pubmed/23145108 http://dx.doi.org/10.1371/journal.pone.0049165 Text en © 2012 Petrie et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Petrie, James R.
Shrestha, Pushkar
Zhou, Xue-Rong
Mansour, Maged P.
Liu, Qing
Belide, Srinivas
Nichols, Peter D.
Singh, Surinder P.
Metabolic Engineering Plant Seeds with Fish Oil-Like Levels of DHA
title Metabolic Engineering Plant Seeds with Fish Oil-Like Levels of DHA
title_full Metabolic Engineering Plant Seeds with Fish Oil-Like Levels of DHA
title_fullStr Metabolic Engineering Plant Seeds with Fish Oil-Like Levels of DHA
title_full_unstemmed Metabolic Engineering Plant Seeds with Fish Oil-Like Levels of DHA
title_short Metabolic Engineering Plant Seeds with Fish Oil-Like Levels of DHA
title_sort metabolic engineering plant seeds with fish oil-like levels of dha
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492320/
https://www.ncbi.nlm.nih.gov/pubmed/23145108
http://dx.doi.org/10.1371/journal.pone.0049165
work_keys_str_mv AT petriejamesr metabolicengineeringplantseedswithfishoillikelevelsofdha
AT shresthapushkar metabolicengineeringplantseedswithfishoillikelevelsofdha
AT zhouxuerong metabolicengineeringplantseedswithfishoillikelevelsofdha
AT mansourmagedp metabolicengineeringplantseedswithfishoillikelevelsofdha
AT liuqing metabolicengineeringplantseedswithfishoillikelevelsofdha
AT belidesrinivas metabolicengineeringplantseedswithfishoillikelevelsofdha
AT nicholspeterd metabolicengineeringplantseedswithfishoillikelevelsofdha
AT singhsurinderp metabolicengineeringplantseedswithfishoillikelevelsofdha