Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila

Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discrim...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramdya, Pavan, Schaffter, Thomas, Floreano, Dario, Benton, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492344/
https://www.ncbi.nlm.nih.gov/pubmed/23144871
http://dx.doi.org/10.1371/journal.pone.0048381
_version_ 1782249114236354560
author Ramdya, Pavan
Schaffter, Thomas
Floreano, Dario
Benton, Richard
author_facet Ramdya, Pavan
Schaffter, Thomas
Floreano, Dario
Benton, Richard
author_sort Ramdya, Pavan
collection PubMed
description Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.
format Online
Article
Text
id pubmed-3492344
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34923442012-11-09 Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila Ramdya, Pavan Schaffter, Thomas Floreano, Dario Benton, Richard PLoS One Research Article Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila. Public Library of Science 2012-11-07 /pmc/articles/PMC3492344/ /pubmed/23144871 http://dx.doi.org/10.1371/journal.pone.0048381 Text en © 2012 Ramdya et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ramdya, Pavan
Schaffter, Thomas
Floreano, Dario
Benton, Richard
Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila
title Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila
title_full Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila
title_fullStr Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila
title_full_unstemmed Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila
title_short Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila
title_sort fluorescence behavioral imaging (fbi) tracks identity in heterogeneous groups of drosophila
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492344/
https://www.ncbi.nlm.nih.gov/pubmed/23144871
http://dx.doi.org/10.1371/journal.pone.0048381
work_keys_str_mv AT ramdyapavan fluorescencebehavioralimagingfbitracksidentityinheterogeneousgroupsofdrosophila
AT schaffterthomas fluorescencebehavioralimagingfbitracksidentityinheterogeneousgroupsofdrosophila
AT floreanodario fluorescencebehavioralimagingfbitracksidentityinheterogeneousgroupsofdrosophila
AT bentonrichard fluorescencebehavioralimagingfbitracksidentityinheterogeneousgroupsofdrosophila

Ejemplares similares