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Water Stress and Aphid Feeding Differentially Influence Metabolite Composition in Arabidopsis thaliana (L.)
Little is known about how drought stress influences plant secondary metabolite accumulation and how this affects plant defense against different aphids. We therefore cultivated Arabidopsis thaliana (L.) plants under well-watered, drought, and water-logged conditions. Two aphid species were selected...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492492/ https://www.ncbi.nlm.nih.gov/pubmed/23144921 http://dx.doi.org/10.1371/journal.pone.0048661 |
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author | Mewis, Inga Khan, Mohammed A. M. Glawischnig, Erich Schreiner, Monika Ulrichs, Christian |
author_facet | Mewis, Inga Khan, Mohammed A. M. Glawischnig, Erich Schreiner, Monika Ulrichs, Christian |
author_sort | Mewis, Inga |
collection | PubMed |
description | Little is known about how drought stress influences plant secondary metabolite accumulation and how this affects plant defense against different aphids. We therefore cultivated Arabidopsis thaliana (L.) plants under well-watered, drought, and water-logged conditions. Two aphid species were selected for this study: the generalist Myzus persicae (Sulzer) and the crucifer specialist Brevicoryne brassicae (L.). Metabolite concentrations in the phloem sap, which influence aphid growth, changed particularly under drought stress. Levels of sucrose and several amino acids, such as glutamic acid, proline, isoleucine, and lysine increased, while concentrations of 4-methoxyindol-3-ylmethyl glucosinolate decreased. M. persicae population growth was highest on plants under drought stress conditions. However, B. brassicae did not profit from improved phloem sap quality under drought stress and performed equally in all water treatments. Water stress and aphids generally had an opposite effect on the accumulation of secondary metabolites in the plant rosettes. Drought stress and water-logging led to increased aliphatic glucosinolate and flavonoid levels. Conversely, aphid feeding, especially of M. persicae, reduced levels of flavonoids and glucosinolates in the plants. Correspondingly, transcript levels of aliphatic biosynthetic genes decreased after feeding of both aphid species. Contrary to M. persicae, drought stress did not promote population growth of B. brassicae on these plants. The specialist aphid induced expression of CYP79B2, CYP79B3, and PAD3 with corresponding accumulation of indolyl glucosinolates and camalexin. This was distinct from M. persicae, which did not elicit similarly strong camalexin accumulation, which led to the hypothesis of a specific defense adaptations against the specialist aphid. |
format | Online Article Text |
id | pubmed-3492492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34924922012-11-09 Water Stress and Aphid Feeding Differentially Influence Metabolite Composition in Arabidopsis thaliana (L.) Mewis, Inga Khan, Mohammed A. M. Glawischnig, Erich Schreiner, Monika Ulrichs, Christian PLoS One Research Article Little is known about how drought stress influences plant secondary metabolite accumulation and how this affects plant defense against different aphids. We therefore cultivated Arabidopsis thaliana (L.) plants under well-watered, drought, and water-logged conditions. Two aphid species were selected for this study: the generalist Myzus persicae (Sulzer) and the crucifer specialist Brevicoryne brassicae (L.). Metabolite concentrations in the phloem sap, which influence aphid growth, changed particularly under drought stress. Levels of sucrose and several amino acids, such as glutamic acid, proline, isoleucine, and lysine increased, while concentrations of 4-methoxyindol-3-ylmethyl glucosinolate decreased. M. persicae population growth was highest on plants under drought stress conditions. However, B. brassicae did not profit from improved phloem sap quality under drought stress and performed equally in all water treatments. Water stress and aphids generally had an opposite effect on the accumulation of secondary metabolites in the plant rosettes. Drought stress and water-logging led to increased aliphatic glucosinolate and flavonoid levels. Conversely, aphid feeding, especially of M. persicae, reduced levels of flavonoids and glucosinolates in the plants. Correspondingly, transcript levels of aliphatic biosynthetic genes decreased after feeding of both aphid species. Contrary to M. persicae, drought stress did not promote population growth of B. brassicae on these plants. The specialist aphid induced expression of CYP79B2, CYP79B3, and PAD3 with corresponding accumulation of indolyl glucosinolates and camalexin. This was distinct from M. persicae, which did not elicit similarly strong camalexin accumulation, which led to the hypothesis of a specific defense adaptations against the specialist aphid. Public Library of Science 2012-11-07 /pmc/articles/PMC3492492/ /pubmed/23144921 http://dx.doi.org/10.1371/journal.pone.0048661 Text en © 2012 Mewis et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Mewis, Inga Khan, Mohammed A. M. Glawischnig, Erich Schreiner, Monika Ulrichs, Christian Water Stress and Aphid Feeding Differentially Influence Metabolite Composition in Arabidopsis thaliana (L.) |
title | Water Stress and Aphid Feeding Differentially Influence Metabolite Composition in Arabidopsis thaliana (L.) |
title_full | Water Stress and Aphid Feeding Differentially Influence Metabolite Composition in Arabidopsis thaliana (L.) |
title_fullStr | Water Stress and Aphid Feeding Differentially Influence Metabolite Composition in Arabidopsis thaliana (L.) |
title_full_unstemmed | Water Stress and Aphid Feeding Differentially Influence Metabolite Composition in Arabidopsis thaliana (L.) |
title_short | Water Stress and Aphid Feeding Differentially Influence Metabolite Composition in Arabidopsis thaliana (L.) |
title_sort | water stress and aphid feeding differentially influence metabolite composition in arabidopsis thaliana (l.) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492492/ https://www.ncbi.nlm.nih.gov/pubmed/23144921 http://dx.doi.org/10.1371/journal.pone.0048661 |
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