Cargando…
Genetic Responses Induced in Olive Roots upon Colonization by the Biocontrol Endophytic Bacterium Pseudomonas fluorescens PICF7
Knowledge on the genetic basis underlying interactions between beneficial bacteria and woody plants is still very limited, and totally absent in the case of olive. We aimed to elucidate genetic responses taking place during the colonization of olive roots by the native endophyte Pseudomonas fluoresc...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492495/ https://www.ncbi.nlm.nih.gov/pubmed/23144916 http://dx.doi.org/10.1371/journal.pone.0048646 |
_version_ | 1782249145058197504 |
---|---|
author | Schilirò, Elisabetta Ferrara, Massimo Nigro, Franco Mercado-Blanco, Jesús |
author_facet | Schilirò, Elisabetta Ferrara, Massimo Nigro, Franco Mercado-Blanco, Jesús |
author_sort | Schilirò, Elisabetta |
collection | PubMed |
description | Knowledge on the genetic basis underlying interactions between beneficial bacteria and woody plants is still very limited, and totally absent in the case of olive. We aimed to elucidate genetic responses taking place during the colonization of olive roots by the native endophyte Pseudomonas fluorescens PICF7, an effective biocontrol agent against Verticillium wilt of olive. Roots of olive plants grown under non-gnotobiotic conditions were collected at different time points after PICF7 inoculation. A Suppression Subtractive Hybridization cDNA library enriched in induced genes was generated. Quantitative real time PCR (qRT-PCR) analysis validated the induction of selected olive genes. Computational analysis of 445 olive ESTs showed that plant defence and response to different stresses represented nearly 45% of genes induced in PICF7-colonized olive roots. Moreover, quantitative real-time PCR (qRT-PCR) analysis confirmed induction of lipoxygenase, phenylpropanoid, terpenoids and plant hormones biosynthesis transcripts. Different classes of transcription factors (i.e., bHLH, WRKYs, GRAS1) were also induced. This work highlights for the first time the ability of an endophytic Pseudomonas spp. strain to mount a wide array of defence responses in an economically-relevant woody crop such as olive, helping to explain its biocontrol activity. |
format | Online Article Text |
id | pubmed-3492495 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34924952012-11-09 Genetic Responses Induced in Olive Roots upon Colonization by the Biocontrol Endophytic Bacterium Pseudomonas fluorescens PICF7 Schilirò, Elisabetta Ferrara, Massimo Nigro, Franco Mercado-Blanco, Jesús PLoS One Research Article Knowledge on the genetic basis underlying interactions between beneficial bacteria and woody plants is still very limited, and totally absent in the case of olive. We aimed to elucidate genetic responses taking place during the colonization of olive roots by the native endophyte Pseudomonas fluorescens PICF7, an effective biocontrol agent against Verticillium wilt of olive. Roots of olive plants grown under non-gnotobiotic conditions were collected at different time points after PICF7 inoculation. A Suppression Subtractive Hybridization cDNA library enriched in induced genes was generated. Quantitative real time PCR (qRT-PCR) analysis validated the induction of selected olive genes. Computational analysis of 445 olive ESTs showed that plant defence and response to different stresses represented nearly 45% of genes induced in PICF7-colonized olive roots. Moreover, quantitative real-time PCR (qRT-PCR) analysis confirmed induction of lipoxygenase, phenylpropanoid, terpenoids and plant hormones biosynthesis transcripts. Different classes of transcription factors (i.e., bHLH, WRKYs, GRAS1) were also induced. This work highlights for the first time the ability of an endophytic Pseudomonas spp. strain to mount a wide array of defence responses in an economically-relevant woody crop such as olive, helping to explain its biocontrol activity. Public Library of Science 2012-11-07 /pmc/articles/PMC3492495/ /pubmed/23144916 http://dx.doi.org/10.1371/journal.pone.0048646 Text en © 2012 Schilirò et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Schilirò, Elisabetta Ferrara, Massimo Nigro, Franco Mercado-Blanco, Jesús Genetic Responses Induced in Olive Roots upon Colonization by the Biocontrol Endophytic Bacterium Pseudomonas fluorescens PICF7 |
title | Genetic Responses Induced in Olive Roots upon Colonization by the Biocontrol Endophytic Bacterium Pseudomonas fluorescens PICF7 |
title_full | Genetic Responses Induced in Olive Roots upon Colonization by the Biocontrol Endophytic Bacterium Pseudomonas fluorescens PICF7 |
title_fullStr | Genetic Responses Induced in Olive Roots upon Colonization by the Biocontrol Endophytic Bacterium Pseudomonas fluorescens PICF7 |
title_full_unstemmed | Genetic Responses Induced in Olive Roots upon Colonization by the Biocontrol Endophytic Bacterium Pseudomonas fluorescens PICF7 |
title_short | Genetic Responses Induced in Olive Roots upon Colonization by the Biocontrol Endophytic Bacterium Pseudomonas fluorescens PICF7 |
title_sort | genetic responses induced in olive roots upon colonization by the biocontrol endophytic bacterium pseudomonas fluorescens picf7 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492495/ https://www.ncbi.nlm.nih.gov/pubmed/23144916 http://dx.doi.org/10.1371/journal.pone.0048646 |
work_keys_str_mv | AT schiliroelisabetta geneticresponsesinducedinoliverootsuponcolonizationbythebiocontrolendophyticbacteriumpseudomonasfluorescenspicf7 AT ferraramassimo geneticresponsesinducedinoliverootsuponcolonizationbythebiocontrolendophyticbacteriumpseudomonasfluorescenspicf7 AT nigrofranco geneticresponsesinducedinoliverootsuponcolonizationbythebiocontrolendophyticbacteriumpseudomonasfluorescenspicf7 AT mercadoblancojesus geneticresponsesinducedinoliverootsuponcolonizationbythebiocontrolendophyticbacteriumpseudomonasfluorescenspicf7 |