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DNA Barcoding of Fish, Insects, and Shellfish in Korea

DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects...

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Autores principales: Kim, Dae-Won, Yoo, Won Gi, Park, Hyun Chul, Yoo, Hye Sook, Kang, Dong Won, Jin, Seon Deok, Min, Hong Ki, Paek, Woon Kee, Lim, Jeongheui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korea Genome Organization 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492657/
https://www.ncbi.nlm.nih.gov/pubmed/23166532
http://dx.doi.org/10.5808/GI.2012.10.3.206
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author Kim, Dae-Won
Yoo, Won Gi
Park, Hyun Chul
Yoo, Hye Sook
Kang, Dong Won
Jin, Seon Deok
Min, Hong Ki
Paek, Woon Kee
Lim, Jeongheui
author_facet Kim, Dae-Won
Yoo, Won Gi
Park, Hyun Chul
Yoo, Hye Sook
Kang, Dong Won
Jin, Seon Deok
Min, Hong Ki
Paek, Woon Kee
Lim, Jeongheui
author_sort Kim, Dae-Won
collection PubMed
description DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects, and shellfish. We present results on phylogenetic relationships to assess biodiversity the in the Korean peninsula. Average GC% contents of the 68 fish species (46.9%), the 59 shellfish species (38.0%), and the 29 insect species (33.2%) are reported. Using the Kimura 2 parameter in all possible pairwise comparisons, the average interspecific distances were compared with the average intraspecific distances in fish (3.22 vs. 0.41), insects (2.06 vs. 0.25), and shellfish (3.58 vs. 0.14). Our results confirm that distance-based DNA barcoding provides sufficient information to identify and delineate fish, insect, and shellfish species by means of all possible pairwise comparisons. These results also confirm that the development of an effective molecular barcode identification system is possible. All DNA barcode sequences collected from our study will be useful for the interpretation of species-level identification and community-level patterns in fish, insects, and shellfish in Korea, although at the species level, the rate of correct identification in a diversified environment might be low.
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spelling pubmed-34926572012-11-19 DNA Barcoding of Fish, Insects, and Shellfish in Korea Kim, Dae-Won Yoo, Won Gi Park, Hyun Chul Yoo, Hye Sook Kang, Dong Won Jin, Seon Deok Min, Hong Ki Paek, Woon Kee Lim, Jeongheui Genomics Inform Original Article DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects, and shellfish. We present results on phylogenetic relationships to assess biodiversity the in the Korean peninsula. Average GC% contents of the 68 fish species (46.9%), the 59 shellfish species (38.0%), and the 29 insect species (33.2%) are reported. Using the Kimura 2 parameter in all possible pairwise comparisons, the average interspecific distances were compared with the average intraspecific distances in fish (3.22 vs. 0.41), insects (2.06 vs. 0.25), and shellfish (3.58 vs. 0.14). Our results confirm that distance-based DNA barcoding provides sufficient information to identify and delineate fish, insect, and shellfish species by means of all possible pairwise comparisons. These results also confirm that the development of an effective molecular barcode identification system is possible. All DNA barcode sequences collected from our study will be useful for the interpretation of species-level identification and community-level patterns in fish, insects, and shellfish in Korea, although at the species level, the rate of correct identification in a diversified environment might be low. Korea Genome Organization 2012-09 2012-09-28 /pmc/articles/PMC3492657/ /pubmed/23166532 http://dx.doi.org/10.5808/GI.2012.10.3.206 Text en Copyright © 2012 by The Korea Genome Organization http://creativecommons.org/licenses/by-nc/3.0 It is identical to the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).
spellingShingle Original Article
Kim, Dae-Won
Yoo, Won Gi
Park, Hyun Chul
Yoo, Hye Sook
Kang, Dong Won
Jin, Seon Deok
Min, Hong Ki
Paek, Woon Kee
Lim, Jeongheui
DNA Barcoding of Fish, Insects, and Shellfish in Korea
title DNA Barcoding of Fish, Insects, and Shellfish in Korea
title_full DNA Barcoding of Fish, Insects, and Shellfish in Korea
title_fullStr DNA Barcoding of Fish, Insects, and Shellfish in Korea
title_full_unstemmed DNA Barcoding of Fish, Insects, and Shellfish in Korea
title_short DNA Barcoding of Fish, Insects, and Shellfish in Korea
title_sort dna barcoding of fish, insects, and shellfish in korea
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492657/
https://www.ncbi.nlm.nih.gov/pubmed/23166532
http://dx.doi.org/10.5808/GI.2012.10.3.206
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