Cargando…

P-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow

Lung cancer is a severe disease threatening human health worldwide. Distant hematogenous metastasis results in poor prognosis and death of lung cancer patients. In the present study, we investigated the effect of circulatory platelets (PLTs) on hematogenous metastasis of non-small cell lung carcinom...

Descripción completa

Detalles Bibliográficos
Autores principales: GONG, LIANG, MI, HONG-JIAN, ZHU, HONGCHU, ZHOU, XIANGDONG, YANG, HEPING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493105/
https://www.ncbi.nlm.nih.gov/pubmed/22266541
http://dx.doi.org/10.3892/mmr.2012.760
_version_ 1782249216516554752
author GONG, LIANG
MI, HONG-JIAN
ZHU, HONGCHU
ZHOU, XIANGDONG
YANG, HEPING
author_facet GONG, LIANG
MI, HONG-JIAN
ZHU, HONGCHU
ZHOU, XIANGDONG
YANG, HEPING
author_sort GONG, LIANG
collection PubMed
description Lung cancer is a severe disease threatening human health worldwide. Distant hematogenous metastasis results in poor prognosis and death of lung cancer patients. In the present study, we investigated the effect of circulatory platelets (PLTs) on hematogenous metastasis of non-small cell lung carcinoma (NSCLC). Laser scanning confocal microscopy was employed to assay the expression of P-selectin in lung cancer tissue, paracancerous tissue and distant tissue, respectively. Meanwhile, fluorescence-activated cell sorting (FACS) was used to determine P-selectin activation in peripheral blood. Purified PLTs were co-cultured with A549 cells and human vascular endothelial cells (HuvECs). Subsequently, the formation of PLT-lung cancer cell complexes and their effects on rolling and adhesion of A549 on the surface of vascular endothelium were assayed. Integrin α3, α5, β1, ICAM-1 and VCAM-1 mRNAs and proteins were measured by reverse RT-PCR and western blot analysis, respectively. The expression of P-selectin in lung adenocarcinoma tissue was significantly stronger compared to that in paracancerous and distant tissues. P-selectin activation in peripheral blood in lung adenocarcinoma was markedly enhanced. The rolling rate of A549 on HuvECs was significantly slowed down after co-culture of activated PLTs and A549 cells. The mRNA and protein levels of integrin α3, α5, β1, ICAM-1 and VCAM-1 were significantly increased after the co-culture. In conclusion, the PLT-lung cancer cell complexes protected the lung cancer cells from mechanical injury under blood flow. Furthermore, up-regulated integrin α3, α5, β1 and endothelial cell adhesion molecules ICAM-1 and VCAM-1 promoted the adhesion of A549 on vascular endothelial cells, which may be responsible for hematogenous metastasis of lung cancer.
format Online
Article
Text
id pubmed-3493105
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-34931052013-04-01 P-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow GONG, LIANG MI, HONG-JIAN ZHU, HONGCHU ZHOU, XIANGDONG YANG, HEPING Mol Med Rep Article Lung cancer is a severe disease threatening human health worldwide. Distant hematogenous metastasis results in poor prognosis and death of lung cancer patients. In the present study, we investigated the effect of circulatory platelets (PLTs) on hematogenous metastasis of non-small cell lung carcinoma (NSCLC). Laser scanning confocal microscopy was employed to assay the expression of P-selectin in lung cancer tissue, paracancerous tissue and distant tissue, respectively. Meanwhile, fluorescence-activated cell sorting (FACS) was used to determine P-selectin activation in peripheral blood. Purified PLTs were co-cultured with A549 cells and human vascular endothelial cells (HuvECs). Subsequently, the formation of PLT-lung cancer cell complexes and their effects on rolling and adhesion of A549 on the surface of vascular endothelium were assayed. Integrin α3, α5, β1, ICAM-1 and VCAM-1 mRNAs and proteins were measured by reverse RT-PCR and western blot analysis, respectively. The expression of P-selectin in lung adenocarcinoma tissue was significantly stronger compared to that in paracancerous and distant tissues. P-selectin activation in peripheral blood in lung adenocarcinoma was markedly enhanced. The rolling rate of A549 on HuvECs was significantly slowed down after co-culture of activated PLTs and A549 cells. The mRNA and protein levels of integrin α3, α5, β1, ICAM-1 and VCAM-1 were significantly increased after the co-culture. In conclusion, the PLT-lung cancer cell complexes protected the lung cancer cells from mechanical injury under blood flow. Furthermore, up-regulated integrin α3, α5, β1 and endothelial cell adhesion molecules ICAM-1 and VCAM-1 promoted the adhesion of A549 on vascular endothelial cells, which may be responsible for hematogenous metastasis of lung cancer. D.A. Spandidos 2012-01-19 2012-04 /pmc/articles/PMC3493105/ /pubmed/22266541 http://dx.doi.org/10.3892/mmr.2012.760 Text en Copyright © 2012, Spandidos Publications
spellingShingle Article
GONG, LIANG
MI, HONG-JIAN
ZHU, HONGCHU
ZHOU, XIANGDONG
YANG, HEPING
P-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow
title P-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow
title_full P-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow
title_fullStr P-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow
title_full_unstemmed P-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow
title_short P-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow
title_sort p-selectin-mediated platelet activation promotes adhesion of non-small cell lung carcinoma cells on vascular endothelial cells under flow
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493105/
https://www.ncbi.nlm.nih.gov/pubmed/22266541
http://dx.doi.org/10.3892/mmr.2012.760
work_keys_str_mv AT gongliang pselectinmediatedplateletactivationpromotesadhesionofnonsmallcelllungcarcinomacellsonvascularendothelialcellsunderflow
AT mihongjian pselectinmediatedplateletactivationpromotesadhesionofnonsmallcelllungcarcinomacellsonvascularendothelialcellsunderflow
AT zhuhongchu pselectinmediatedplateletactivationpromotesadhesionofnonsmallcelllungcarcinomacellsonvascularendothelialcellsunderflow
AT zhouxiangdong pselectinmediatedplateletactivationpromotesadhesionofnonsmallcelllungcarcinomacellsonvascularendothelialcellsunderflow
AT yangheping pselectinmediatedplateletactivationpromotesadhesionofnonsmallcelllungcarcinomacellsonvascularendothelialcellsunderflow