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4D shear stress maps of the developing heart using Doppler optical coherence tomography

Accurate imaging and measurement of hemodynamic forces is vital for investigating how physical forces acting on the embryonic heart are transduced and influence developmental pathways. Of particular importance is blood flow-induced shear stress, which influences gene expression by endothelial cells...

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Autores principales: Peterson, Lindsy M., Jenkins, Michael W., Gu, Shi, Barwick, Lee, Watanabe, Michiko, Rollins, Andrew M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493225/
https://www.ncbi.nlm.nih.gov/pubmed/23162737
http://dx.doi.org/10.1364/BOE.3.003022
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author Peterson, Lindsy M.
Jenkins, Michael W.
Gu, Shi
Barwick, Lee
Watanabe, Michiko
Rollins, Andrew M.
author_facet Peterson, Lindsy M.
Jenkins, Michael W.
Gu, Shi
Barwick, Lee
Watanabe, Michiko
Rollins, Andrew M.
author_sort Peterson, Lindsy M.
collection PubMed
description Accurate imaging and measurement of hemodynamic forces is vital for investigating how physical forces acting on the embryonic heart are transduced and influence developmental pathways. Of particular importance is blood flow-induced shear stress, which influences gene expression by endothelial cells and potentially leads to congenital heart defects through abnormal heart looping, septation, and valvulogenesis. However no imaging tool has been available to measure shear stress on the endocardium volumetrically and dynamically. Using 4D structural and Doppler OCT imaging, we are able to accurately measure the blood flow in the heart tube in vivo and to map endocardial shear stress throughout the heart cycle under physiological conditions for the first time. These measurements of the shear stress patterns will enable precise titration of experimental perturbations and accurate correlation of shear with the expression of molecules critical to heart development.
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spelling pubmed-34932252012-11-16 4D shear stress maps of the developing heart using Doppler optical coherence tomography Peterson, Lindsy M. Jenkins, Michael W. Gu, Shi Barwick, Lee Watanabe, Michiko Rollins, Andrew M. Biomed Opt Express Optical Coherence Tomography Accurate imaging and measurement of hemodynamic forces is vital for investigating how physical forces acting on the embryonic heart are transduced and influence developmental pathways. Of particular importance is blood flow-induced shear stress, which influences gene expression by endothelial cells and potentially leads to congenital heart defects through abnormal heart looping, septation, and valvulogenesis. However no imaging tool has been available to measure shear stress on the endocardium volumetrically and dynamically. Using 4D structural and Doppler OCT imaging, we are able to accurately measure the blood flow in the heart tube in vivo and to map endocardial shear stress throughout the heart cycle under physiological conditions for the first time. These measurements of the shear stress patterns will enable precise titration of experimental perturbations and accurate correlation of shear with the expression of molecules critical to heart development. Optical Society of America 2012-10-31 /pmc/articles/PMC3493225/ /pubmed/23162737 http://dx.doi.org/10.1364/BOE.3.003022 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Optical Coherence Tomography
Peterson, Lindsy M.
Jenkins, Michael W.
Gu, Shi
Barwick, Lee
Watanabe, Michiko
Rollins, Andrew M.
4D shear stress maps of the developing heart using Doppler optical coherence tomography
title 4D shear stress maps of the developing heart using Doppler optical coherence tomography
title_full 4D shear stress maps of the developing heart using Doppler optical coherence tomography
title_fullStr 4D shear stress maps of the developing heart using Doppler optical coherence tomography
title_full_unstemmed 4D shear stress maps of the developing heart using Doppler optical coherence tomography
title_short 4D shear stress maps of the developing heart using Doppler optical coherence tomography
title_sort 4d shear stress maps of the developing heart using doppler optical coherence tomography
topic Optical Coherence Tomography
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493225/
https://www.ncbi.nlm.nih.gov/pubmed/23162737
http://dx.doi.org/10.1364/BOE.3.003022
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