Genetic PEGylation

Polyethylene glycol (PEG) was genetically incorporated into a polypeptide. Stop-anticodon-containing tRNAs were acylated with PEG-containing amino acids and were then translated into polypeptides corresponding to DNA sequences containing the stop codons. The molecular weights of the PEG used were 17...

Descripción completa

Detalles Bibliográficos
Autores principales: Tada, Seiichi, Andou, Takashi, Suzuki, Takehiro, Dohmae, Naoshi, Kobatake, Eiry, Ito, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493536/
https://www.ncbi.nlm.nih.gov/pubmed/23145132
http://dx.doi.org/10.1371/journal.pone.0049235
_version_ 1782249282023194624
author Tada, Seiichi
Andou, Takashi
Suzuki, Takehiro
Dohmae, Naoshi
Kobatake, Eiry
Ito, Yoshihiro
author_facet Tada, Seiichi
Andou, Takashi
Suzuki, Takehiro
Dohmae, Naoshi
Kobatake, Eiry
Ito, Yoshihiro
author_sort Tada, Seiichi
collection PubMed
description Polyethylene glycol (PEG) was genetically incorporated into a polypeptide. Stop-anticodon-containing tRNAs were acylated with PEG-containing amino acids and were then translated into polypeptides corresponding to DNA sequences containing the stop codons. The molecular weights of the PEG used were 170, 500, 700, 1000, and 2000 Da, and the translation was confirmed by mass spectrometry. The PEG incorporation ratio decreased as the molecular weight of PEG increased, and PEG with a molecular weight of 1000 Da was only slightly incorporated. Although improvement is required to increase the efficiency of the process, this study demonstrates the possibility of genetic PEGylation.
format Online
Article
Text
id pubmed-3493536
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34935362012-11-09 Genetic PEGylation Tada, Seiichi Andou, Takashi Suzuki, Takehiro Dohmae, Naoshi Kobatake, Eiry Ito, Yoshihiro PLoS One Research Article Polyethylene glycol (PEG) was genetically incorporated into a polypeptide. Stop-anticodon-containing tRNAs were acylated with PEG-containing amino acids and were then translated into polypeptides corresponding to DNA sequences containing the stop codons. The molecular weights of the PEG used were 170, 500, 700, 1000, and 2000 Da, and the translation was confirmed by mass spectrometry. The PEG incorporation ratio decreased as the molecular weight of PEG increased, and PEG with a molecular weight of 1000 Da was only slightly incorporated. Although improvement is required to increase the efficiency of the process, this study demonstrates the possibility of genetic PEGylation. Public Library of Science 2012-11-08 /pmc/articles/PMC3493536/ /pubmed/23145132 http://dx.doi.org/10.1371/journal.pone.0049235 Text en © 2012 Tada et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tada, Seiichi
Andou, Takashi
Suzuki, Takehiro
Dohmae, Naoshi
Kobatake, Eiry
Ito, Yoshihiro
Genetic PEGylation
title Genetic PEGylation
title_full Genetic PEGylation
title_fullStr Genetic PEGylation
title_full_unstemmed Genetic PEGylation
title_short Genetic PEGylation
title_sort genetic pegylation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493536/
https://www.ncbi.nlm.nih.gov/pubmed/23145132
http://dx.doi.org/10.1371/journal.pone.0049235
work_keys_str_mv AT tadaseiichi geneticpegylation
AT andoutakashi geneticpegylation
AT suzukitakehiro geneticpegylation
AT dohmaenaoshi geneticpegylation
AT kobatakeeiry geneticpegylation
AT itoyoshihiro geneticpegylation

Ejemplares similares