Promoter and histone methylation and p16(INK4A) gene expression in colon cancer
The inactivation of the cyclin-dependent kinase inhibitor p16(INK4A) gene by hypermethylation is observed in numerous types of cancer. New findings indicate that DNA and histone methylation act in concert in gene silencing. In this study, we investigated the methylation status of the p16(INK4A) gene...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493785/ https://www.ncbi.nlm.nih.gov/pubmed/23226740 http://dx.doi.org/10.3892/etm.2012.683 |
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author | YORUKER, EBRU ESIN MERT, UFUK BUGRA, DURSUN YAMANER, SUMER DALAY, NEJAT |
author_facet | YORUKER, EBRU ESIN MERT, UFUK BUGRA, DURSUN YAMANER, SUMER DALAY, NEJAT |
author_sort | YORUKER, EBRU ESIN |
collection | PubMed |
description | The inactivation of the cyclin-dependent kinase inhibitor p16(INK4A) gene by hypermethylation is observed in numerous types of cancer. New findings indicate that DNA and histone methylation act in concert in gene silencing. In this study, we investigated the methylation status of the p16(INK4A) gene promoter and the histone 3 lysine 9 residue in the tumors and matched normal tissue samples from patients with colorectal cancer and analyzed their association with gene expression. The methylation and expression of the p16(INK4A) gene were analyzed by real-time PCR, and histone methylation was analyzed by chromatin immunoprecipitation followed by real-time PCR. p16(INK4A) expression was significantly higher in the tumors compared to normal tissue. Mono-, di- and trimethylation levels of the H3K9 residue were similar in the tumor and normal tissue samples. We did not observe any significant correlation between p16(INK4A) methylation or expression and clinical parameters. Our results suggest that epigenetic modifications of the p16(INK4A) gene and histone lysine methylation do not play a major role in colon carcinogenesis. |
format | Online Article Text |
id | pubmed-3493785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-34937852012-12-06 Promoter and histone methylation and p16(INK4A) gene expression in colon cancer YORUKER, EBRU ESIN MERT, UFUK BUGRA, DURSUN YAMANER, SUMER DALAY, NEJAT Exp Ther Med Articles The inactivation of the cyclin-dependent kinase inhibitor p16(INK4A) gene by hypermethylation is observed in numerous types of cancer. New findings indicate that DNA and histone methylation act in concert in gene silencing. In this study, we investigated the methylation status of the p16(INK4A) gene promoter and the histone 3 lysine 9 residue in the tumors and matched normal tissue samples from patients with colorectal cancer and analyzed their association with gene expression. The methylation and expression of the p16(INK4A) gene were analyzed by real-time PCR, and histone methylation was analyzed by chromatin immunoprecipitation followed by real-time PCR. p16(INK4A) expression was significantly higher in the tumors compared to normal tissue. Mono-, di- and trimethylation levels of the H3K9 residue were similar in the tumor and normal tissue samples. We did not observe any significant correlation between p16(INK4A) methylation or expression and clinical parameters. Our results suggest that epigenetic modifications of the p16(INK4A) gene and histone lysine methylation do not play a major role in colon carcinogenesis. D.A. Spandidos 2012-11 2012-08-24 /pmc/articles/PMC3493785/ /pubmed/23226740 http://dx.doi.org/10.3892/etm.2012.683 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles YORUKER, EBRU ESIN MERT, UFUK BUGRA, DURSUN YAMANER, SUMER DALAY, NEJAT Promoter and histone methylation and p16(INK4A) gene expression in colon cancer |
title | Promoter and histone methylation and p16(INK4A) gene expression in colon cancer |
title_full | Promoter and histone methylation and p16(INK4A) gene expression in colon cancer |
title_fullStr | Promoter and histone methylation and p16(INK4A) gene expression in colon cancer |
title_full_unstemmed | Promoter and histone methylation and p16(INK4A) gene expression in colon cancer |
title_short | Promoter and histone methylation and p16(INK4A) gene expression in colon cancer |
title_sort | promoter and histone methylation and p16(ink4a) gene expression in colon cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493785/ https://www.ncbi.nlm.nih.gov/pubmed/23226740 http://dx.doi.org/10.3892/etm.2012.683 |
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