Cargando…

Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis

The aim of this study was to identify immunoreactive neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP) neurons in the autonomic and sensory ganglia, specifically neurons that innervate the rat temporomandibular joint (TMJ). A possible variation between the percentages of these neurons...

Descripción completa

Detalles Bibliográficos
Autores principales: Damico, J.P., Ervolino, E., Torres, K.R., Batagello, D.S., Cruz-Rizzolo, R.J., Casatti, C.A., Bauer, J.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493977/
https://www.ncbi.nlm.nih.gov/pubmed/23027347
http://dx.doi.org/10.4081/ejh.2012.e31
_version_ 1782249346555707392
author Damico, J.P.
Ervolino, E.
Torres, K.R.
Batagello, D.S.
Cruz-Rizzolo, R.J.
Casatti, C.A.
Bauer, J.A.
author_facet Damico, J.P.
Ervolino, E.
Torres, K.R.
Batagello, D.S.
Cruz-Rizzolo, R.J.
Casatti, C.A.
Bauer, J.A.
author_sort Damico, J.P.
collection PubMed
description The aim of this study was to identify immunoreactive neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP) neurons in the autonomic and sensory ganglia, specifically neurons that innervate the rat temporomandibular joint (TMJ). A possible variation between the percentages of these neurons in acute and chronic phases of carrageenan-induced arthritis was examined. Retrograde neuronal tracing was combined with indirect immunofluorescence to identify NPY-immunoreactive (NPY-IR) and CGRP- immunoreactive (CGRP-IR) neurons that send nerve fibers to the normal and arthritic temporomandibular joint. In normal joints, NPY-IR neurons constitute 78±3%, 77±6% and 10±4% of double-labeled nucleated neuronal profile originated from the superior cervical, stellate and otic ganglia, respectively. These percentages in the sympathetic ganglia were significantly decreased in acute (58±2% for superior cervical ganglion and 58±8% for stellate ganglion) and chronic (60±2% for superior cervical ganglion and 59±15% for stellate ganglion) phases of arthritis, while in the otic ganglion these percentages were significantly increased to 19±5% and 13±3%, respectively. In the trigeminal ganglion, CGRP-IR neurons innervating the joint significantly increased from 31±3% in normal animals to 54±2% and 49±3% in the acute and chronic phases of arthritis, respectively. It can be concluded that NPY neurons that send nerve fibers to the rat temporomandibular joint are located mainly in the superior cervical, stellate and otic ganglia. Acute and chronic phases of carrageenan-induced arthritis lead to an increase in the percentage of NPY-IR parasympathetic and CGRP-IR sensory neurons and to a decrease in the percentage of NPY-IR sympathetic neurons related to TMJ innervation.
format Online
Article
Text
id pubmed-3493977
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher PAGEPress Publications
record_format MEDLINE/PubMed
spelling pubmed-34939772012-11-09 Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis Damico, J.P. Ervolino, E. Torres, K.R. Batagello, D.S. Cruz-Rizzolo, R.J. Casatti, C.A. Bauer, J.A. Eur J Histochem Original Paper The aim of this study was to identify immunoreactive neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP) neurons in the autonomic and sensory ganglia, specifically neurons that innervate the rat temporomandibular joint (TMJ). A possible variation between the percentages of these neurons in acute and chronic phases of carrageenan-induced arthritis was examined. Retrograde neuronal tracing was combined with indirect immunofluorescence to identify NPY-immunoreactive (NPY-IR) and CGRP- immunoreactive (CGRP-IR) neurons that send nerve fibers to the normal and arthritic temporomandibular joint. In normal joints, NPY-IR neurons constitute 78±3%, 77±6% and 10±4% of double-labeled nucleated neuronal profile originated from the superior cervical, stellate and otic ganglia, respectively. These percentages in the sympathetic ganglia were significantly decreased in acute (58±2% for superior cervical ganglion and 58±8% for stellate ganglion) and chronic (60±2% for superior cervical ganglion and 59±15% for stellate ganglion) phases of arthritis, while in the otic ganglion these percentages were significantly increased to 19±5% and 13±3%, respectively. In the trigeminal ganglion, CGRP-IR neurons innervating the joint significantly increased from 31±3% in normal animals to 54±2% and 49±3% in the acute and chronic phases of arthritis, respectively. It can be concluded that NPY neurons that send nerve fibers to the rat temporomandibular joint are located mainly in the superior cervical, stellate and otic ganglia. Acute and chronic phases of carrageenan-induced arthritis lead to an increase in the percentage of NPY-IR parasympathetic and CGRP-IR sensory neurons and to a decrease in the percentage of NPY-IR sympathetic neurons related to TMJ innervation. PAGEPress Publications 2012-10-01 /pmc/articles/PMC3493977/ /pubmed/23027347 http://dx.doi.org/10.4081/ejh.2012.e31 Text en ©Copyright J.P. Damico et al., 2012 This work is licensed under a Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0). Licensee PAGEPress, Italy
spellingShingle Original Paper
Damico, J.P.
Ervolino, E.
Torres, K.R.
Batagello, D.S.
Cruz-Rizzolo, R.J.
Casatti, C.A.
Bauer, J.A.
Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis
title Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis
title_full Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis
title_fullStr Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis
title_full_unstemmed Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis
title_short Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis
title_sort phenotypic alterations of neuropeptide y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493977/
https://www.ncbi.nlm.nih.gov/pubmed/23027347
http://dx.doi.org/10.4081/ejh.2012.e31
work_keys_str_mv AT damicojp phenotypicalterationsofneuropeptideyandcalcitoningenerelatedpeptidecontainingneuronsinnervatingtherattemporomandibularjointduringcarrageenaninducedarthritis
AT ervolinoe phenotypicalterationsofneuropeptideyandcalcitoningenerelatedpeptidecontainingneuronsinnervatingtherattemporomandibularjointduringcarrageenaninducedarthritis
AT torreskr phenotypicalterationsofneuropeptideyandcalcitoningenerelatedpeptidecontainingneuronsinnervatingtherattemporomandibularjointduringcarrageenaninducedarthritis
AT batagellods phenotypicalterationsofneuropeptideyandcalcitoningenerelatedpeptidecontainingneuronsinnervatingtherattemporomandibularjointduringcarrageenaninducedarthritis
AT cruzrizzolorj phenotypicalterationsofneuropeptideyandcalcitoningenerelatedpeptidecontainingneuronsinnervatingtherattemporomandibularjointduringcarrageenaninducedarthritis
AT casattica phenotypicalterationsofneuropeptideyandcalcitoningenerelatedpeptidecontainingneuronsinnervatingtherattemporomandibularjointduringcarrageenaninducedarthritis
AT bauerja phenotypicalterationsofneuropeptideyandcalcitoningenerelatedpeptidecontainingneuronsinnervatingtherattemporomandibularjointduringcarrageenaninducedarthritis