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Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome

The aim of this study was to assess the genetic status of cagA, vacA subtype and iceA genotypes of Helicobacter pylori and the relationship with upper gastrointestinal diseases in Northeast China. Gastric biopsies were obtained from 378 patients with upper gastrointestinal diseases and 197 samples w...

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Autores principales: WEI, GUO-CHAO, CHEN, JING, LIU, AI-YUN, ZHANG, MIAO, LIU, XIAO-JUN, LIU, DAN, XU, JUN, LIU, BING-RONG, LING, HONG, WU, HUA-XING, DU, YA-JU
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3494117/
https://www.ncbi.nlm.nih.gov/pubmed/23226771
http://dx.doi.org/10.3892/etm.2012.704
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author WEI, GUO-CHAO
CHEN, JING
LIU, AI-YUN
ZHANG, MIAO
LIU, XIAO-JUN
LIU, DAN
XU, JUN
LIU, BING-RONG
LING, HONG
WU, HUA-XING
DU, YA-JU
author_facet WEI, GUO-CHAO
CHEN, JING
LIU, AI-YUN
ZHANG, MIAO
LIU, XIAO-JUN
LIU, DAN
XU, JUN
LIU, BING-RONG
LING, HONG
WU, HUA-XING
DU, YA-JU
author_sort WEI, GUO-CHAO
collection PubMed
description The aim of this study was to assess the genetic status of cagA, vacA subtype and iceA genotypes of Helicobacter pylori and the relationship with upper gastrointestinal diseases in Northeast China. Gastric biopsies were obtained from 378 patients with upper gastrointestinal diseases and 197 samples were used. The cagA, vacA alleles and iceA genotypes were determined by polymerase chain reaction. CagA was present in 176 (89.3%) of 197 patients. Of the 197 cases, 186 (94.4%) had vacA signal sequence s1c allele, 6 (3%) had s1a and 5 (2.5%) had s1b. The vacA s2 genotype was not detected in our study. VacA middle region sequences, m1 and m2, were found in 20 (10.2%) and 150 (76.1%), respectively. The allelic variant iceA1 (70.1%) was more prevalent than iceA2 (23.4%). The vacA allele s1am2 had a significant relationship with the presence of gastric cancer (p<0.05) and the iceA1 genotype was also associated with gastric cancer (p<0.05). These may be useful risk factors for upper gastrointestinal diseases.
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spelling pubmed-34941172012-12-06 Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome WEI, GUO-CHAO CHEN, JING LIU, AI-YUN ZHANG, MIAO LIU, XIAO-JUN LIU, DAN XU, JUN LIU, BING-RONG LING, HONG WU, HUA-XING DU, YA-JU Exp Ther Med Articles The aim of this study was to assess the genetic status of cagA, vacA subtype and iceA genotypes of Helicobacter pylori and the relationship with upper gastrointestinal diseases in Northeast China. Gastric biopsies were obtained from 378 patients with upper gastrointestinal diseases and 197 samples were used. The cagA, vacA alleles and iceA genotypes were determined by polymerase chain reaction. CagA was present in 176 (89.3%) of 197 patients. Of the 197 cases, 186 (94.4%) had vacA signal sequence s1c allele, 6 (3%) had s1a and 5 (2.5%) had s1b. The vacA s2 genotype was not detected in our study. VacA middle region sequences, m1 and m2, were found in 20 (10.2%) and 150 (76.1%), respectively. The allelic variant iceA1 (70.1%) was more prevalent than iceA2 (23.4%). The vacA allele s1am2 had a significant relationship with the presence of gastric cancer (p<0.05) and the iceA1 genotype was also associated with gastric cancer (p<0.05). These may be useful risk factors for upper gastrointestinal diseases. D.A. Spandidos 2012-12 2012-09-11 /pmc/articles/PMC3494117/ /pubmed/23226771 http://dx.doi.org/10.3892/etm.2012.704 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
WEI, GUO-CHAO
CHEN, JING
LIU, AI-YUN
ZHANG, MIAO
LIU, XIAO-JUN
LIU, DAN
XU, JUN
LIU, BING-RONG
LING, HONG
WU, HUA-XING
DU, YA-JU
Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome
title Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome
title_full Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome
title_fullStr Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome
title_full_unstemmed Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome
title_short Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome
title_sort prevalence of helicobacter pylori vaca, caga and icea genotypes and correlation with clinical outcome
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3494117/
https://www.ncbi.nlm.nih.gov/pubmed/23226771
http://dx.doi.org/10.3892/etm.2012.704
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