Cargando…

Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores

We have compared the performance of two Troponin-C-based calcium FRET sensors using fluorescence lifetime read-outs. The first sensor, TN-L15, consists of a Troponin-C fragment inserted between CFP and Citrine while the second sensor, called mTFP-TnC-Cit, was realized by replacing CFP in TN-L15 with...

Descripción completa

Detalles Bibliográficos
Autores principales:	Laine, Romain, Stuckey, Daniel W., Manning, Hugh, Warren, Sean C., Kennedy, Gordon, Carling, David, Dunsby, Chris, Sardini, Alessandro, French, Paul M. W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3494685/ https://www.ncbi.nlm.nih.gov/pubmed/23152874 http://dx.doi.org/10.1371/journal.pone.0049200

Ejemplares similares

DARPins recognize mTFP1 as novel reagents for in vitro and in vivo protein manipulations
por: Vigano, M. Alessandra, et al.
Publicado: (2018)

Genetically Encoded, pH-Sensitive mTFP1 Biosensor for Probing Lysosomal pH
por: Chin, Marcus Y., et al.
Publicado: (2021)

Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse
por: Chitirala, Praneeth, et al.
Publicado: (2020)

In vivo fluorescence lifetime tomography of a FRET probe expressed in mouse
por: McGinty, James, et al.
Publicado: (2011)

Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM
por: Chennell, George, et al.
Publicado: (2016)

Correction: DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations (doi:10.1242/bio.036749)
por: Vigano, M. Alessandra, et al.
Publicado: (2018)

FLIM FRET Technology for Drug Discovery: Automated Multiwell-Plate High-Content Analysis, Multiplexed Readouts and Application in Situ**
por: Kumar, Sunil, et al.
Publicado: (2011)

3-Chlorodiphenylamine activates cardiac troponin by a mechanism distinct from bepridil or TFP
por: Tikunova, Svetlana B., et al.
Publicado: (2019)

Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)
por: Margineanu, Anca, et al.
Publicado: (2016)

Corrigendum: Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)
por: Margineanu, Anca, et al.
Publicado: (2016)

A Method to Quantify FRET Stoichiometry with Phasor Plot Analysis and Acceptor Lifetime Ingrowth
por: Chen, WeiYue, et al.
Publicado: (2015)

Quantitative analysis of recombination between YFP and CFP genes of FRET biosensors introduced by lentiviral or retroviral gene transfer
por: Komatsubara, Akira T., et al.
Publicado: (2015)

Homo-FRET Based Biosensors and Their Application to Multiplexed Imaging of Signalling Events in Live Cells
por: Warren, Sean C., et al.
Publicado: (2015)

Repositioning of the antipsychotic drug TFP for sepsis treatment
por: Park, Jung Hwa, et al.
Publicado: (2019)

Does the Digital Economy Increase Green TFP in Cities?
por: Zhao, Chuanyu, et al.
Publicado: (2023)

Automated fluorescence lifetime imaging plate reader and its application to Förster resonant energy transfer readout of Gag protein aggregation
por: Alibhai, Dominic, et al.
Publicado: (2013)

Development of Low-Cost Instrumentation for Single Point Autofluorescence Lifetime Measurements
por: Lagarto, João, et al.
Publicado: (2017)

Correction Approach for Delta Function Convolution Model Fitting of Fluorescence Decay Data in the Case of a Monoexponential Reference Fluorophore
por: Talbot, Clifford B., et al.
Publicado: (2015)

Fluorescence lifetime predicts performance of voltage sensitive fluorophores in cardiomyocytes and neurons
por: Boggess, Steven C., et al.
Publicado: (2020)

Simultaneous readout of multiple FRET pairs using photochromism
por: Roebroek, Thijs, et al.
Publicado: (2021)

FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis
por: Kim, Jiho, et al.
Publicado: (2017)

in vivo quantitative FRET small animal imaging: intensity versus lifetime-based FRET
por: Smith, Jason T., et al.
Publicado: (2023)

In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET
por: Smith, Jason T., et al.
Publicado: (2023)

Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells
por: Glöckner, Nina, et al.
Publicado: (2022)

TFP Bioeconomy Impact post Covid-19 on the agricultural economy
por: Zuniga-Gonzalez, C. A.
Publicado: (2023)

Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates
por: Maioli, Vincent, et al.
Publicado: (2016)

Knockdown of Mtfp1 can minimize doxorubicin cardiotoxicity by inhibiting Dnm1l‐mediated mitochondrial fission
por: Aung, Lynn H. H., et al.
Publicado: (2017)

Transcriptome Profiling of Developing Ovine Fat Tail Tissue Reveals an Important Role for MTFP1 in Regulation of Adipogenesis
por: Han, Jiangang, et al.
Publicado: (2022)

Time-resolved FRET reports FGFR1 dimerization and formation of a complex with its effector PLCγ1
por: Perdios, Louis, et al.
Publicado: (2016)

Determination of tumor necrosis factor receptor-associated factor trimerization in living cells by CFP→YFP→mRFP FRET detected by flow cytometry
por: He, Liusheng, et al.
Publicado: (2005)

Quantification of Small Molecule–Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore
por: Lee, Molly M., et al.
Publicado: (2016)

Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc
por: Aper, Stijn J. A., et al.
Publicado: (2016)

How does firms' broadband adoption affect regional TFP in Italy?
por: Giannini, Massimo, et al.
Publicado: (2022)

Mercury poisoning caused by Chinese folk prescription (CFP): A case report and analysis of both CFP and quackery
por: Mo, Tingting, et al.
Publicado: (2016)

Förster resonance energy transfer: Role of diffusion of fluorophore orientation and separation in observed shifts of FRET efficiency
por: Wallace, Bram, et al.
Publicado: (2017)

Preparation of site-specifically fluorophore-labeled polyubiquitin chains for FRET studies of Cdc48 substrate processing
por: Williams, Cameron, et al.
Publicado: (2023)

What drives TFP long-run dynamics in five large European economies?
por: Bellocchi, Alessandro, et al.
Publicado: (2021)

Molecular Dynamics Simulations of the Cardiac Troponin Complex Performed with FRET Distances as Restraints
por: Jayasundar, Jayant James, et al.
Publicado: (2014)

Precise fluorophore lifetime mapping in live-cell, multi-photon excitation microscopy
por: Chang, Ching-Wei, et al.
Publicado: (2010)

Lifetime-based analysis of binary fluorophores mixtures in the low photon count limit
por: Nasser, Maisa, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_03bsi7qgp45lcte16hdves7h51