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SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors

Primary cilia serve as cellular antenna for various sensory signaling pathways. However, how the sensory receptors are properly targeted to the ciliary surface remains poorly understood. Here, we show that UBC-9, the sole E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme, physically interac...

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Detalles Bibliográficos
Autores principales: Li, Yujie, Zhang, Qing, Wei, Qing, Zhang, Yuxia, Ling, Kun, Hu, Jinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3494855/
https://www.ncbi.nlm.nih.gov/pubmed/23128241
http://dx.doi.org/10.1083/jcb.201203150
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author Li, Yujie
Zhang, Qing
Wei, Qing
Zhang, Yuxia
Ling, Kun
Hu, Jinghua
author_facet Li, Yujie
Zhang, Qing
Wei, Qing
Zhang, Yuxia
Ling, Kun
Hu, Jinghua
author_sort Li, Yujie
collection PubMed
description Primary cilia serve as cellular antenna for various sensory signaling pathways. However, how the sensory receptors are properly targeted to the ciliary surface remains poorly understood. Here, we show that UBC-9, the sole E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme, physically interacts with and SUMOylates the C terminus of small GTPase ARL-13, the worm orthologue of ARL13B that mutated in ciliopathy Joubert syndrome. Mutations that totally abolish the SUMOylation of ARL-13 do not affect its established role in ciliogenesis, but fail to regulate the proper ciliary targeting of various sensory receptors and consequently compromise the corresponding sensory functions. Conversely, constitutively SUMOylated ARL-13 fully rescues all ciliary defects of arl-13–null animals. Furthermore, SUMOylation modification of human ARL13B is required for the ciliary entry of polycystin-2, the protein mutated in autosomal dominant polycystic kidney disease. Our data reveal a novel but conserved role for the SUMOylation modification of ciliary small GTPase ARL13B in specifically regulating the proper ciliary targeting of various sensory receptors.
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spelling pubmed-34948552013-05-12 SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors Li, Yujie Zhang, Qing Wei, Qing Zhang, Yuxia Ling, Kun Hu, Jinghua J Cell Biol Research Articles Primary cilia serve as cellular antenna for various sensory signaling pathways. However, how the sensory receptors are properly targeted to the ciliary surface remains poorly understood. Here, we show that UBC-9, the sole E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme, physically interacts with and SUMOylates the C terminus of small GTPase ARL-13, the worm orthologue of ARL13B that mutated in ciliopathy Joubert syndrome. Mutations that totally abolish the SUMOylation of ARL-13 do not affect its established role in ciliogenesis, but fail to regulate the proper ciliary targeting of various sensory receptors and consequently compromise the corresponding sensory functions. Conversely, constitutively SUMOylated ARL-13 fully rescues all ciliary defects of arl-13–null animals. Furthermore, SUMOylation modification of human ARL13B is required for the ciliary entry of polycystin-2, the protein mutated in autosomal dominant polycystic kidney disease. Our data reveal a novel but conserved role for the SUMOylation modification of ciliary small GTPase ARL13B in specifically regulating the proper ciliary targeting of various sensory receptors. The Rockefeller University Press 2012-11-12 /pmc/articles/PMC3494855/ /pubmed/23128241 http://dx.doi.org/10.1083/jcb.201203150 Text en © 2012 Li et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Li, Yujie
Zhang, Qing
Wei, Qing
Zhang, Yuxia
Ling, Kun
Hu, Jinghua
SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors
title SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors
title_full SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors
title_fullStr SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors
title_full_unstemmed SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors
title_short SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors
title_sort sumoylation of the small gtpase arl-13 promotes ciliary targeting of sensory receptors
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3494855/
https://www.ncbi.nlm.nih.gov/pubmed/23128241
http://dx.doi.org/10.1083/jcb.201203150
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