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Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae
BACKGROUND: Recent successes in the determination of G-protein coupled receptor (GPCR) structures have relied on the ability of receptor variants to overcome difficulties in expression and purification. Therefore, the quick screening of functionally expressed stable receptor variants is vital. RESUL...
Autores principales: | Shiroishi, Mitsunori, Tsujimoto, Hirokazu, Makyio, Hisayoshi, Asada, Hidetsugu, Yurugi-Kobayashi, Takami, Shimamura, Tatsuro, Murata, Takeshi, Nomura, Norimichi, Haga, Tatsuya, Iwata, So, Kobayashi, Takuya |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495400/ https://www.ncbi.nlm.nih.gov/pubmed/22694812 http://dx.doi.org/10.1186/1475-2859-11-78 |
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