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Effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo

BACKGROUND: Helium inhalation protects myocardium, brain and endothelium against ischemia/reperfusion injury in animals and humans, when applied according to specific “conditioning” protocols. Before widespread use of this “conditioning” agent in clinical practice, negative side effects have to be r...

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Autores principales: Oei, Gezina TML, Smit, Kirsten F, vd Vondervoort, Djai, Brevoord, Daniel, Hoogendijk, Arjan, Wieland, Catharina W, Hollmann, Markus W, Preckel, Benedikt, Weber, Nina C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495766/
https://www.ncbi.nlm.nih.gov/pubmed/23006534
http://dx.doi.org/10.1186/1479-5876-10-201
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author Oei, Gezina TML
Smit, Kirsten F
vd Vondervoort, Djai
Brevoord, Daniel
Hoogendijk, Arjan
Wieland, Catharina W
Hollmann, Markus W
Preckel, Benedikt
Weber, Nina C
author_facet Oei, Gezina TML
Smit, Kirsten F
vd Vondervoort, Djai
Brevoord, Daniel
Hoogendijk, Arjan
Wieland, Catharina W
Hollmann, Markus W
Preckel, Benedikt
Weber, Nina C
author_sort Oei, Gezina TML
collection PubMed
description BACKGROUND: Helium inhalation protects myocardium, brain and endothelium against ischemia/reperfusion injury in animals and humans, when applied according to specific “conditioning” protocols. Before widespread use of this “conditioning” agent in clinical practice, negative side effects have to be ruled out. We investigated the effect of prolonged helium inhalation on the responsiveness of the human immune response in whole blood ex vivo. METHODS: Male healthy volunteers inhaled 30 minutes heliox (79%He/21%O(2)) or air in a cross over design, with two weeks between measurements. Blood was withdrawn at T0 (baseline), T1 (25 min inhalation) and T2-T5 (1, 2, 6, 24 h after inhalation) and incubated with lipopolysaccharide (LPS), lipoteichoic acid (LTA), T-cell stimuli anti-CD3/ anti-CD28 (TCS) or RPMI (as control) for 2, 4 and 24 hours or not incubated (0 h). An additional group of six volunteers inhaled 60 minutes of heliox or air, followed by blood incubation with LPS and RPMI. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), interferon-γ (IFN-γ) and interleukin-2 (IL-2) was analyzed by cytometric bead array. Statistical analysis was performed by the Wilcoxon test for matched samples. RESULTS: Incubation with LPS, LTA or TCS significantly increased TNF-α, IL-1β, IL-6, IL-8, IFN-γ and IL-2 in comparison to incubation with RPMI alone. Thirty min of helium inhalation did not influence the amounts of TNF-α, IL-1β, IL-6, IL-8, IFN-γ and IL-2 in comparison to air. Sixty min of helium inhalation did not affect cytokine production after LPS stimulation. CONCLUSIONS: We conclude that 79% helium inhalation does not affect the responsiveness of the human immune system in healthy volunteers. TRIAL REGISTRATION: Dutch Trial Register: http://www.trialregister.nl/ NTR2152
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spelling pubmed-34957662012-11-13 Effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo Oei, Gezina TML Smit, Kirsten F vd Vondervoort, Djai Brevoord, Daniel Hoogendijk, Arjan Wieland, Catharina W Hollmann, Markus W Preckel, Benedikt Weber, Nina C J Transl Med Research BACKGROUND: Helium inhalation protects myocardium, brain and endothelium against ischemia/reperfusion injury in animals and humans, when applied according to specific “conditioning” protocols. Before widespread use of this “conditioning” agent in clinical practice, negative side effects have to be ruled out. We investigated the effect of prolonged helium inhalation on the responsiveness of the human immune response in whole blood ex vivo. METHODS: Male healthy volunteers inhaled 30 minutes heliox (79%He/21%O(2)) or air in a cross over design, with two weeks between measurements. Blood was withdrawn at T0 (baseline), T1 (25 min inhalation) and T2-T5 (1, 2, 6, 24 h after inhalation) and incubated with lipopolysaccharide (LPS), lipoteichoic acid (LTA), T-cell stimuli anti-CD3/ anti-CD28 (TCS) or RPMI (as control) for 2, 4 and 24 hours or not incubated (0 h). An additional group of six volunteers inhaled 60 minutes of heliox or air, followed by blood incubation with LPS and RPMI. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), interferon-γ (IFN-γ) and interleukin-2 (IL-2) was analyzed by cytometric bead array. Statistical analysis was performed by the Wilcoxon test for matched samples. RESULTS: Incubation with LPS, LTA or TCS significantly increased TNF-α, IL-1β, IL-6, IL-8, IFN-γ and IL-2 in comparison to incubation with RPMI alone. Thirty min of helium inhalation did not influence the amounts of TNF-α, IL-1β, IL-6, IL-8, IFN-γ and IL-2 in comparison to air. Sixty min of helium inhalation did not affect cytokine production after LPS stimulation. CONCLUSIONS: We conclude that 79% helium inhalation does not affect the responsiveness of the human immune system in healthy volunteers. TRIAL REGISTRATION: Dutch Trial Register: http://www.trialregister.nl/ NTR2152 BioMed Central 2012-09-24 /pmc/articles/PMC3495766/ /pubmed/23006534 http://dx.doi.org/10.1186/1479-5876-10-201 Text en Copyright ©2012 Oei et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Oei, Gezina TML
Smit, Kirsten F
vd Vondervoort, Djai
Brevoord, Daniel
Hoogendijk, Arjan
Wieland, Catharina W
Hollmann, Markus W
Preckel, Benedikt
Weber, Nina C
Effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo
title Effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo
title_full Effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo
title_fullStr Effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo
title_full_unstemmed Effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo
title_short Effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo
title_sort effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495766/
https://www.ncbi.nlm.nih.gov/pubmed/23006534
http://dx.doi.org/10.1186/1479-5876-10-201
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