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Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products
BACKGROUND: Pyrethroids are widely used for the control of pests and insects, as pyrethroids are believed to pose little risk to human health and environment. However, exposure to the pyrethroids exceeding the label directions might adversely affect human health and environment. Hence a careful sele...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496573/ https://www.ncbi.nlm.nih.gov/pubmed/22937871 http://dx.doi.org/10.1186/1752-153X-6-93 |
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author | Musharraf, Syed Ghulam Shoaib, Muhammad Kumar, Dileep Najam-ul-Haq, Muhammad |
author_facet | Musharraf, Syed Ghulam Shoaib, Muhammad Kumar, Dileep Najam-ul-Haq, Muhammad |
author_sort | Musharraf, Syed Ghulam |
collection | PubMed |
description | BACKGROUND: Pyrethroids are widely used for the control of pests and insects, as pyrethroids are believed to pose little risk to human health and environment. However, exposure to the pyrethroids exceeding the label directions might adversely affect human health and environment. Hence a careful selection of environment friendly household product is required that must contain exactly the label claimed pyrethroids amount. RESULTS: A sensitive and robust TLC-densitometric method for simultaneous quantification of commonly used synthetic pyrethroids including esbiothrin, alpha-cypermethrin and cis/trans permethrin in agricultural and domestic products has been developed and validated. TLC aluminum sheets, precoated with 0.2 mm thick layer of silica gel 60 F-254, were used for chromatographic process. Densitometric analysis of chromatoplates was carried out in absorbance mode at corresponding λ(max) of each pyrethroid. Equally valid common mobile phase for all pyrethroids consisted of hexane-dichloromethane-ethylacetate-formic acid (8:1.5:0.4:0.1 v/v/v/v) which provided sharp and symmetrical peaks of esbiothrin, alpha-cypermethrin, trans-permethrin and cis-permethrin, at R(f) 0.31, 0.53, 0.6 and 0.65, respectively. Linear regression data for respective calibration curves showed a good linearity for all pyrethroids with r = 0.991-0.996. Limits of detection (LOD) and limits of quantification (LOQ) for all pyrethroids were found in the range of 1.6-2.8 and 4.9-8.5 ng/spot, respectively. CONCLUSIONS: The developed method is applicable for separating the mixture of pyrethroids and at the same time, it is also valid for separating their isomers. The method is reproducible, precise and accurate for the quantitative determination of pyrethroids in agricultural and domestic products. |
format | Online Article Text |
id | pubmed-3496573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34965732012-11-14 Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products Musharraf, Syed Ghulam Shoaib, Muhammad Kumar, Dileep Najam-ul-Haq, Muhammad Chem Cent J Research Article BACKGROUND: Pyrethroids are widely used for the control of pests and insects, as pyrethroids are believed to pose little risk to human health and environment. However, exposure to the pyrethroids exceeding the label directions might adversely affect human health and environment. Hence a careful selection of environment friendly household product is required that must contain exactly the label claimed pyrethroids amount. RESULTS: A sensitive and robust TLC-densitometric method for simultaneous quantification of commonly used synthetic pyrethroids including esbiothrin, alpha-cypermethrin and cis/trans permethrin in agricultural and domestic products has been developed and validated. TLC aluminum sheets, precoated with 0.2 mm thick layer of silica gel 60 F-254, were used for chromatographic process. Densitometric analysis of chromatoplates was carried out in absorbance mode at corresponding λ(max) of each pyrethroid. Equally valid common mobile phase for all pyrethroids consisted of hexane-dichloromethane-ethylacetate-formic acid (8:1.5:0.4:0.1 v/v/v/v) which provided sharp and symmetrical peaks of esbiothrin, alpha-cypermethrin, trans-permethrin and cis-permethrin, at R(f) 0.31, 0.53, 0.6 and 0.65, respectively. Linear regression data for respective calibration curves showed a good linearity for all pyrethroids with r = 0.991-0.996. Limits of detection (LOD) and limits of quantification (LOQ) for all pyrethroids were found in the range of 1.6-2.8 and 4.9-8.5 ng/spot, respectively. CONCLUSIONS: The developed method is applicable for separating the mixture of pyrethroids and at the same time, it is also valid for separating their isomers. The method is reproducible, precise and accurate for the quantitative determination of pyrethroids in agricultural and domestic products. BioMed Central 2012-08-31 /pmc/articles/PMC3496573/ /pubmed/22937871 http://dx.doi.org/10.1186/1752-153X-6-93 Text en Copyright ©2012 Musharraf et al.; licensee Chemistry Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Musharraf, Syed Ghulam Shoaib, Muhammad Kumar, Dileep Najam-ul-Haq, Muhammad Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title | Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_full | Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_fullStr | Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_full_unstemmed | Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_short | Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_sort | validated tlc-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496573/ https://www.ncbi.nlm.nih.gov/pubmed/22937871 http://dx.doi.org/10.1186/1752-153X-6-93 |
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