The Impact of Heterogeneity and Dark Acceptor States on FRET: Implications for Using Fluorescent Protein Donors and Acceptors

Förster resonance energy transfer (FRET) microscopy is widely used to study protein interactions in living cells. Typically, spectral variants of the Green Fluorescent Protein (FPs) are incorporated into proteins expressed in cells, and FRET between donor and acceptor FPs is assayed. As appreciable...

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Detalles Bibliográficos
Autores principales: Vogel, Steven S., Nguyen, Tuan A., van der Meer, B. Wieb, Blank, Paul S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496711/
https://www.ncbi.nlm.nih.gov/pubmed/23152925
http://dx.doi.org/10.1371/journal.pone.0049593

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