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A Novel Real-Time PCR Assay of microRNAs Using S-Poly(T), a Specific Oligo(dT) Reverse Transcription Primer with Excellent Sensitivity and Specificity
BACKGROUND: MicroRNAs (miRNAs) are small, non-coding RNAs capable of postranscriptionally regulating gene expression. Accurate expression profiling is crucial for understanding the biological roles of miRNAs, and exploring them as biomarkers of diseases. METHODOLOGY/PRINCIPAL FINDINGS: A novel, high...
Autores principales: | Kang, Kang, Zhang, Xiaoying, Liu, Hongtao, Wang, Zhiwei, Zhong, Jiasheng, Huang, Zhenting, Peng, Xiao, Zeng, Yan, Wang, Yuna, Yang, Yi, Luo, Jun, Gou, Deming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496722/ https://www.ncbi.nlm.nih.gov/pubmed/23152780 http://dx.doi.org/10.1371/journal.pone.0048536 |
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