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Assessment of the Bacteriocinogenic Potential of Marine Bacteria Reveals Lichenicidin Production by Seaweed-Derived Bacillus spp.

The objectives of this study were (1) to assess the bacteriocinogenic potential of bacteria derived mainly from seaweed, but also sand and seawater, (2) to identify at least some of the bacteriocins produced, if any and (3) to determine if they are unique to the marine environment and/or novel. Fift...

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Autores principales: Luz Prieto, Maria, O’Sullivan, Laurie, Tan, Shiau Pin, McLoughlin, Peter, Hughes, Helen, O’Connor, Paula M., Cotter, Paul D., Lawlor, Peadar G., Gardiner, Gillian E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497023/
https://www.ncbi.nlm.nih.gov/pubmed/23170084
http://dx.doi.org/10.3390/md10102280
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author Luz Prieto, Maria
O’Sullivan, Laurie
Tan, Shiau Pin
McLoughlin, Peter
Hughes, Helen
O’Connor, Paula M.
Cotter, Paul D.
Lawlor, Peadar G.
Gardiner, Gillian E.
author_facet Luz Prieto, Maria
O’Sullivan, Laurie
Tan, Shiau Pin
McLoughlin, Peter
Hughes, Helen
O’Connor, Paula M.
Cotter, Paul D.
Lawlor, Peadar G.
Gardiner, Gillian E.
author_sort Luz Prieto, Maria
collection PubMed
description The objectives of this study were (1) to assess the bacteriocinogenic potential of bacteria derived mainly from seaweed, but also sand and seawater, (2) to identify at least some of the bacteriocins produced, if any and (3) to determine if they are unique to the marine environment and/or novel. Fifteen Bacillus licheniformis or pumilus isolates with antimicrobial activity against at least one of the indicator bacteria used were recovered. Some, at least, of the antimicrobials produced were bacteriocins, as they were proteinaceous and the producers displayed immunity. Screening with PCR primers for known Bacillus bacteriocins revealed that three seaweed-derived Bacillus licheniformis harbored the bli04127 gene which encodes one of the peptides of the two-peptide lantibiotic lichenicidin. Production of both lichenicidin peptides was then confirmed by mass spectrometry. This is the first definitive proof of bacteriocin production by seaweed-derived bacteria. The authors acknowledge that the bacteriocin produced has previously been discovered and is not unique to the marine environment. However, the other marine isolates likely produce novel bacteriocins, as none harboured genes for known Bacillus bacteriocins.
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spelling pubmed-34970232012-11-20 Assessment of the Bacteriocinogenic Potential of Marine Bacteria Reveals Lichenicidin Production by Seaweed-Derived Bacillus spp. Luz Prieto, Maria O’Sullivan, Laurie Tan, Shiau Pin McLoughlin, Peter Hughes, Helen O’Connor, Paula M. Cotter, Paul D. Lawlor, Peadar G. Gardiner, Gillian E. Mar Drugs Article The objectives of this study were (1) to assess the bacteriocinogenic potential of bacteria derived mainly from seaweed, but also sand and seawater, (2) to identify at least some of the bacteriocins produced, if any and (3) to determine if they are unique to the marine environment and/or novel. Fifteen Bacillus licheniformis or pumilus isolates with antimicrobial activity against at least one of the indicator bacteria used were recovered. Some, at least, of the antimicrobials produced were bacteriocins, as they were proteinaceous and the producers displayed immunity. Screening with PCR primers for known Bacillus bacteriocins revealed that three seaweed-derived Bacillus licheniformis harbored the bli04127 gene which encodes one of the peptides of the two-peptide lantibiotic lichenicidin. Production of both lichenicidin peptides was then confirmed by mass spectrometry. This is the first definitive proof of bacteriocin production by seaweed-derived bacteria. The authors acknowledge that the bacteriocin produced has previously been discovered and is not unique to the marine environment. However, the other marine isolates likely produce novel bacteriocins, as none harboured genes for known Bacillus bacteriocins. MDPI 2012-10-18 /pmc/articles/PMC3497023/ /pubmed/23170084 http://dx.doi.org/10.3390/md10102280 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Luz Prieto, Maria
O’Sullivan, Laurie
Tan, Shiau Pin
McLoughlin, Peter
Hughes, Helen
O’Connor, Paula M.
Cotter, Paul D.
Lawlor, Peadar G.
Gardiner, Gillian E.
Assessment of the Bacteriocinogenic Potential of Marine Bacteria Reveals Lichenicidin Production by Seaweed-Derived Bacillus spp.
title Assessment of the Bacteriocinogenic Potential of Marine Bacteria Reveals Lichenicidin Production by Seaweed-Derived Bacillus spp.
title_full Assessment of the Bacteriocinogenic Potential of Marine Bacteria Reveals Lichenicidin Production by Seaweed-Derived Bacillus spp.
title_fullStr Assessment of the Bacteriocinogenic Potential of Marine Bacteria Reveals Lichenicidin Production by Seaweed-Derived Bacillus spp.
title_full_unstemmed Assessment of the Bacteriocinogenic Potential of Marine Bacteria Reveals Lichenicidin Production by Seaweed-Derived Bacillus spp.
title_short Assessment of the Bacteriocinogenic Potential of Marine Bacteria Reveals Lichenicidin Production by Seaweed-Derived Bacillus spp.
title_sort assessment of the bacteriocinogenic potential of marine bacteria reveals lichenicidin production by seaweed-derived bacillus spp.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497023/
https://www.ncbi.nlm.nih.gov/pubmed/23170084
http://dx.doi.org/10.3390/md10102280
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