Biocatalytic Synthesis of Poly(δ-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst

The ring-opening polymerization of δ-valerolactone catalyzed by a thermophilic esterase from the archaeon Archaeoglobus fulgidus was successfully conducted in organic solvents. The effects of enzyme concentration, temperature, reaction time and reaction medium on monomer conversion and product molec...

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Detalles Bibliográficos
Autores principales: Cao, Hong, Han, Haobo, Li, Guangquan, Yang, Jiebing, Zhang, Lingfei, Yang, Yan, Fang, Xuedong, Li, Quanshun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497269/
https://www.ncbi.nlm.nih.gov/pubmed/23202895
http://dx.doi.org/10.3390/ijms131012232
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author Cao, Hong
Han, Haobo
Li, Guangquan
Yang, Jiebing
Zhang, Lingfei
Yang, Yan
Fang, Xuedong
Li, Quanshun
author_facet Cao, Hong
Han, Haobo
Li, Guangquan
Yang, Jiebing
Zhang, Lingfei
Yang, Yan
Fang, Xuedong
Li, Quanshun
author_sort Cao, Hong
collection PubMed
description The ring-opening polymerization of δ-valerolactone catalyzed by a thermophilic esterase from the archaeon Archaeoglobus fulgidus was successfully conducted in organic solvents. The effects of enzyme concentration, temperature, reaction time and reaction medium on monomer conversion and product molecular weight were systematically evaluated. Through the optimization of reaction conditions, poly(δ-valerolactone) was produced in 97% monomer conversion, with a number-average molecular weight of 2225 g/mol, in toluene at 70 °C for 72 h. This paper has produced a new biocatalyst for the synthesis of poly(δ-valerolactone), and also deeper insight has been gained into the mechanism of thermophilic esterase-catalyzed ring-opening polymerization.
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spelling pubmed-34972692012-11-29 Biocatalytic Synthesis of Poly(δ-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst Cao, Hong Han, Haobo Li, Guangquan Yang, Jiebing Zhang, Lingfei Yang, Yan Fang, Xuedong Li, Quanshun Int J Mol Sci Article The ring-opening polymerization of δ-valerolactone catalyzed by a thermophilic esterase from the archaeon Archaeoglobus fulgidus was successfully conducted in organic solvents. The effects of enzyme concentration, temperature, reaction time and reaction medium on monomer conversion and product molecular weight were systematically evaluated. Through the optimization of reaction conditions, poly(δ-valerolactone) was produced in 97% monomer conversion, with a number-average molecular weight of 2225 g/mol, in toluene at 70 °C for 72 h. This paper has produced a new biocatalyst for the synthesis of poly(δ-valerolactone), and also deeper insight has been gained into the mechanism of thermophilic esterase-catalyzed ring-opening polymerization. Molecular Diversity Preservation International (MDPI) 2012-09-25 /pmc/articles/PMC3497269/ /pubmed/23202895 http://dx.doi.org/10.3390/ijms131012232 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0).
spellingShingle Article
Cao, Hong
Han, Haobo
Li, Guangquan
Yang, Jiebing
Zhang, Lingfei
Yang, Yan
Fang, Xuedong
Li, Quanshun
Biocatalytic Synthesis of Poly(δ-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst
title Biocatalytic Synthesis of Poly(δ-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst
title_full Biocatalytic Synthesis of Poly(δ-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst
title_fullStr Biocatalytic Synthesis of Poly(δ-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst
title_full_unstemmed Biocatalytic Synthesis of Poly(δ-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst
title_short Biocatalytic Synthesis of Poly(δ-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst
title_sort biocatalytic synthesis of poly(δ-valerolactone) using a thermophilic esterase from archaeoglobus fulgidus as catalyst
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497269/
https://www.ncbi.nlm.nih.gov/pubmed/23202895
http://dx.doi.org/10.3390/ijms131012232
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