Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies

In the present work, we produced two monoclonal antibodies (BrBm37 and BrBm38) and tested their action against the triosephosphate isomerase of Rhipicephalus (Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition...

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Autores principales: Saramago, Luiz, Franceschi, Mariana, Logullo, Carlos, Masuda, Aoi, Vaz, Itabajara da Silva, Farias, Sandra Estrazulas, Moraes, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497315/
https://www.ncbi.nlm.nih.gov/pubmed/23202941
http://dx.doi.org/10.3390/ijms131013118
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author Saramago, Luiz
Franceschi, Mariana
Logullo, Carlos
Masuda, Aoi
Vaz, Itabajara da Silva
Farias, Sandra Estrazulas
Moraes, Jorge
author_facet Saramago, Luiz
Franceschi, Mariana
Logullo, Carlos
Masuda, Aoi
Vaz, Itabajara da Silva
Farias, Sandra Estrazulas
Moraes, Jorge
author_sort Saramago, Luiz
collection PubMed
description In the present work, we produced two monoclonal antibodies (BrBm37 and BrBm38) and tested their action against the triosephosphate isomerase of Rhipicephalus (Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition by BrBm37 was up to 85% whereas that of BrBrm38 was 98%, depending on the antibody-enzyme ratio. RmTIM activity was lower in ovarian, gut, and fat body tissue extracts treated with BrBm37 or BrBm38 mAbs. The proliferation of the embryonic tick cell line (BME26) was inhibited by BrBm37 and BrBm38 mAbs. In summary, the results reveal that it is possible to interfere with the RmTIM function using antibodies, even in intact cells.
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spelling pubmed-34973152012-11-29 Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies Saramago, Luiz Franceschi, Mariana Logullo, Carlos Masuda, Aoi Vaz, Itabajara da Silva Farias, Sandra Estrazulas Moraes, Jorge Int J Mol Sci Article In the present work, we produced two monoclonal antibodies (BrBm37 and BrBm38) and tested their action against the triosephosphate isomerase of Rhipicephalus (Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition by BrBm37 was up to 85% whereas that of BrBrm38 was 98%, depending on the antibody-enzyme ratio. RmTIM activity was lower in ovarian, gut, and fat body tissue extracts treated with BrBm37 or BrBm38 mAbs. The proliferation of the embryonic tick cell line (BME26) was inhibited by BrBm37 and BrBm38 mAbs. In summary, the results reveal that it is possible to interfere with the RmTIM function using antibodies, even in intact cells. Molecular Diversity Preservation International (MDPI) 2012-10-12 /pmc/articles/PMC3497315/ /pubmed/23202941 http://dx.doi.org/10.3390/ijms131013118 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0).
spellingShingle Article
Saramago, Luiz
Franceschi, Mariana
Logullo, Carlos
Masuda, Aoi
Vaz, Itabajara da Silva
Farias, Sandra Estrazulas
Moraes, Jorge
Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies
title Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies
title_full Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies
title_fullStr Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies
title_full_unstemmed Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies
title_short Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies
title_sort inhibition of enzyme activity of rhipicephalus (boophilus) microplus triosephosphate isomerase and bme26 cell growth by monoclonal antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497315/
https://www.ncbi.nlm.nih.gov/pubmed/23202941
http://dx.doi.org/10.3390/ijms131013118
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