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Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints

[Image: see text] Using a surface force balance, normal and shear interactions have been measured between two atomically smooth surfaces coated with hyaluronan (HA), and with HA/aggrecan (Agg) complexes stabilized by cartilage link protein (LP). Such HA/Agg/LP complexes are the most abundant mobile...

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Autores principales: Seror, Jasmine, Merkher, Yulia, Kampf, Nir, Collinson, Lisa, Day, Anthony J., Maroudas, Alice, Klein, Jacob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2012
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497856/
https://www.ncbi.nlm.nih.gov/pubmed/23074968
http://dx.doi.org/10.1021/bm301283f
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author Seror, Jasmine
Merkher, Yulia
Kampf, Nir
Collinson, Lisa
Day, Anthony J.
Maroudas, Alice
Klein, Jacob
author_facet Seror, Jasmine
Merkher, Yulia
Kampf, Nir
Collinson, Lisa
Day, Anthony J.
Maroudas, Alice
Klein, Jacob
author_sort Seror, Jasmine
collection PubMed
description [Image: see text] Using a surface force balance, normal and shear interactions have been measured between two atomically smooth surfaces coated with hyaluronan (HA), and with HA/aggrecan (Agg) complexes stabilized by cartilage link protein (LP). Such HA/Agg/LP complexes are the most abundant mobile macromolecular species permeating articular cartilage in synovial joints and have been conjectured to be present as boundary lubricants at its surface. The aim of the present study is to gain insight into the extremely efficient lubrication when two cartilage surfaces slide past each other in healthy joints, and in particular to elucidate the possible role in this of the HA/Agg/LP complexes. Within the range of our parameters, our results reveal that the HA/Agg/LP macromolecular surface complexes are much better boundary lubricants than HA alone, likely because of the higher level of hydration, due to the higher charge density, of the HA/Agg/LP layers with respect to the HA alone. However, the friction coefficients (μ) associated with the mutual interactions and sliding of opposing HA/Agg/LP layers (μ ≈ 0.01 up to pressure P of ca. 12 atm, increasing sharply at higher P) suggest that such complexes by themselves cannot account for the remarkable boundary lubrication observed in mammalian joints (up to P > 50 atm).
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spelling pubmed-34978562012-11-15 Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints Seror, Jasmine Merkher, Yulia Kampf, Nir Collinson, Lisa Day, Anthony J. Maroudas, Alice Klein, Jacob Biomacromolecules [Image: see text] Using a surface force balance, normal and shear interactions have been measured between two atomically smooth surfaces coated with hyaluronan (HA), and with HA/aggrecan (Agg) complexes stabilized by cartilage link protein (LP). Such HA/Agg/LP complexes are the most abundant mobile macromolecular species permeating articular cartilage in synovial joints and have been conjectured to be present as boundary lubricants at its surface. The aim of the present study is to gain insight into the extremely efficient lubrication when two cartilage surfaces slide past each other in healthy joints, and in particular to elucidate the possible role in this of the HA/Agg/LP complexes. Within the range of our parameters, our results reveal that the HA/Agg/LP macromolecular surface complexes are much better boundary lubricants than HA alone, likely because of the higher level of hydration, due to the higher charge density, of the HA/Agg/LP layers with respect to the HA alone. However, the friction coefficients (μ) associated with the mutual interactions and sliding of opposing HA/Agg/LP layers (μ ≈ 0.01 up to pressure P of ca. 12 atm, increasing sharply at higher P) suggest that such complexes by themselves cannot account for the remarkable boundary lubrication observed in mammalian joints (up to P > 50 atm). American Chemical Society 2012-10-17 2012-11-12 /pmc/articles/PMC3497856/ /pubmed/23074968 http://dx.doi.org/10.1021/bm301283f Text en Copyright © 2012 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle Seror, Jasmine
Merkher, Yulia
Kampf, Nir
Collinson, Lisa
Day, Anthony J.
Maroudas, Alice
Klein, Jacob
Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints
title Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints
title_full Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints
title_fullStr Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints
title_full_unstemmed Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints
title_short Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints
title_sort normal and shear interactions between hyaluronan–aggrecan complexes mimicking possible boundary lubricants in articular cartilage in synovial joints
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497856/
https://www.ncbi.nlm.nih.gov/pubmed/23074968
http://dx.doi.org/10.1021/bm301283f
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