Cargando…
Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints
[Image: see text] Using a surface force balance, normal and shear interactions have been measured between two atomically smooth surfaces coated with hyaluronan (HA), and with HA/aggrecan (Agg) complexes stabilized by cartilage link protein (LP). Such HA/Agg/LP complexes are the most abundant mobile...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2012
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497856/ https://www.ncbi.nlm.nih.gov/pubmed/23074968 http://dx.doi.org/10.1021/bm301283f |
_version_ | 1782249765373739008 |
---|---|
author | Seror, Jasmine Merkher, Yulia Kampf, Nir Collinson, Lisa Day, Anthony J. Maroudas, Alice Klein, Jacob |
author_facet | Seror, Jasmine Merkher, Yulia Kampf, Nir Collinson, Lisa Day, Anthony J. Maroudas, Alice Klein, Jacob |
author_sort | Seror, Jasmine |
collection | PubMed |
description | [Image: see text] Using a surface force balance, normal and shear interactions have been measured between two atomically smooth surfaces coated with hyaluronan (HA), and with HA/aggrecan (Agg) complexes stabilized by cartilage link protein (LP). Such HA/Agg/LP complexes are the most abundant mobile macromolecular species permeating articular cartilage in synovial joints and have been conjectured to be present as boundary lubricants at its surface. The aim of the present study is to gain insight into the extremely efficient lubrication when two cartilage surfaces slide past each other in healthy joints, and in particular to elucidate the possible role in this of the HA/Agg/LP complexes. Within the range of our parameters, our results reveal that the HA/Agg/LP macromolecular surface complexes are much better boundary lubricants than HA alone, likely because of the higher level of hydration, due to the higher charge density, of the HA/Agg/LP layers with respect to the HA alone. However, the friction coefficients (μ) associated with the mutual interactions and sliding of opposing HA/Agg/LP layers (μ ≈ 0.01 up to pressure P of ca. 12 atm, increasing sharply at higher P) suggest that such complexes by themselves cannot account for the remarkable boundary lubrication observed in mammalian joints (up to P > 50 atm). |
format | Online Article Text |
id | pubmed-3497856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-34978562012-11-15 Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints Seror, Jasmine Merkher, Yulia Kampf, Nir Collinson, Lisa Day, Anthony J. Maroudas, Alice Klein, Jacob Biomacromolecules [Image: see text] Using a surface force balance, normal and shear interactions have been measured between two atomically smooth surfaces coated with hyaluronan (HA), and with HA/aggrecan (Agg) complexes stabilized by cartilage link protein (LP). Such HA/Agg/LP complexes are the most abundant mobile macromolecular species permeating articular cartilage in synovial joints and have been conjectured to be present as boundary lubricants at its surface. The aim of the present study is to gain insight into the extremely efficient lubrication when two cartilage surfaces slide past each other in healthy joints, and in particular to elucidate the possible role in this of the HA/Agg/LP complexes. Within the range of our parameters, our results reveal that the HA/Agg/LP macromolecular surface complexes are much better boundary lubricants than HA alone, likely because of the higher level of hydration, due to the higher charge density, of the HA/Agg/LP layers with respect to the HA alone. However, the friction coefficients (μ) associated with the mutual interactions and sliding of opposing HA/Agg/LP layers (μ ≈ 0.01 up to pressure P of ca. 12 atm, increasing sharply at higher P) suggest that such complexes by themselves cannot account for the remarkable boundary lubrication observed in mammalian joints (up to P > 50 atm). American Chemical Society 2012-10-17 2012-11-12 /pmc/articles/PMC3497856/ /pubmed/23074968 http://dx.doi.org/10.1021/bm301283f Text en Copyright © 2012 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org. |
spellingShingle | Seror, Jasmine Merkher, Yulia Kampf, Nir Collinson, Lisa Day, Anthony J. Maroudas, Alice Klein, Jacob Normal and Shear Interactions between Hyaluronan–Aggrecan Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage in Synovial Joints |
title | Normal and Shear Interactions
between Hyaluronan–Aggrecan
Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage
in Synovial Joints |
title_full | Normal and Shear Interactions
between Hyaluronan–Aggrecan
Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage
in Synovial Joints |
title_fullStr | Normal and Shear Interactions
between Hyaluronan–Aggrecan
Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage
in Synovial Joints |
title_full_unstemmed | Normal and Shear Interactions
between Hyaluronan–Aggrecan
Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage
in Synovial Joints |
title_short | Normal and Shear Interactions
between Hyaluronan–Aggrecan
Complexes Mimicking Possible Boundary Lubricants in Articular Cartilage
in Synovial Joints |
title_sort | normal and shear interactions
between hyaluronan–aggrecan
complexes mimicking possible boundary lubricants in articular cartilage
in synovial joints |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497856/ https://www.ncbi.nlm.nih.gov/pubmed/23074968 http://dx.doi.org/10.1021/bm301283f |
work_keys_str_mv | AT serorjasmine normalandshearinteractionsbetweenhyaluronanaggrecancomplexesmimickingpossibleboundarylubricantsinarticularcartilageinsynovialjoints AT merkheryulia normalandshearinteractionsbetweenhyaluronanaggrecancomplexesmimickingpossibleboundarylubricantsinarticularcartilageinsynovialjoints AT kampfnir normalandshearinteractionsbetweenhyaluronanaggrecancomplexesmimickingpossibleboundarylubricantsinarticularcartilageinsynovialjoints AT collinsonlisa normalandshearinteractionsbetweenhyaluronanaggrecancomplexesmimickingpossibleboundarylubricantsinarticularcartilageinsynovialjoints AT dayanthonyj normalandshearinteractionsbetweenhyaluronanaggrecancomplexesmimickingpossibleboundarylubricantsinarticularcartilageinsynovialjoints AT maroudasalice normalandshearinteractionsbetweenhyaluronanaggrecancomplexesmimickingpossibleboundarylubricantsinarticularcartilageinsynovialjoints AT kleinjacob normalandshearinteractionsbetweenhyaluronanaggrecancomplexesmimickingpossibleboundarylubricantsinarticularcartilageinsynovialjoints |