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Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton

BACKGROUND: Previous transcriptome profiling studies have investigated the molecular mechanisms of pollen and anther development, and identified many genes involved in these processes. However, only 51 anther ESTs of Upland cotton (Gossypium hirsutum) were found in NCBI and there have been no report...

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Autores principales: Ma, Jianhui, Wei, Hengling, Song, Meizhen, Pang, Chaoyou, Liu, Ji, Wang, Long, Zhang, Jinfa, Fan, Shuli, Yu, Shuxun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498337/
https://www.ncbi.nlm.nih.gov/pubmed/23155472
http://dx.doi.org/10.1371/journal.pone.0049244
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author Ma, Jianhui
Wei, Hengling
Song, Meizhen
Pang, Chaoyou
Liu, Ji
Wang, Long
Zhang, Jinfa
Fan, Shuli
Yu, Shuxun
author_facet Ma, Jianhui
Wei, Hengling
Song, Meizhen
Pang, Chaoyou
Liu, Ji
Wang, Long
Zhang, Jinfa
Fan, Shuli
Yu, Shuxun
author_sort Ma, Jianhui
collection PubMed
description BACKGROUND: Previous transcriptome profiling studies have investigated the molecular mechanisms of pollen and anther development, and identified many genes involved in these processes. However, only 51 anther ESTs of Upland cotton (Gossypium hirsutum) were found in NCBI and there have been no reports of transcriptome profiling analyzing anther development in Upland cotton, a major fiber crop in the word. METHODOLOGY/PRINCIPAL FINDING: Ninety-eight hundred and ninety-six high quality ESTs were sequenced from their 3′-ends and assembled into 6,643 unigenes from a normalized, full-length anther cDNA library of Upland cotton. Combined with previous sequenced anther-related ESTs, 12,244 unigenes were generated as the reference genes for digital gene expression (DGE) analysis. The DGE was conducted on anthers that were isolated at tetrad pollen (TTP), uninucleate pollen (UNP), binucleate pollen (BNP) and mature pollen (MTP) periods along with four other tissues, i.e., roots (RO), stems (ST), leaves (LV) and embryos (EB). Through transcriptome profiling analysis, we identified 1,165 genes that were enriched at certain anther development periods, and many of them were involved in starch and sucrose metabolism, pentose and glucuronate interconversion, flavonoid biosynthesis, and ascorbate and aldarate metabolism. CONCLUSIONS/SIGNIFICANCE: We first generated a normalized, full-length cDNA library from anthers and performed transcriptome profiling analysis of anther development in Upland cotton. From these results, 10,178 anther expressed genes were identified, among which 1,165 genes were stage-enriched in anthers. And many of these stage-enriched genes were involved in some important processes regulating anther development.
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spelling pubmed-34983372012-11-15 Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton Ma, Jianhui Wei, Hengling Song, Meizhen Pang, Chaoyou Liu, Ji Wang, Long Zhang, Jinfa Fan, Shuli Yu, Shuxun PLoS One Research Article BACKGROUND: Previous transcriptome profiling studies have investigated the molecular mechanisms of pollen and anther development, and identified many genes involved in these processes. However, only 51 anther ESTs of Upland cotton (Gossypium hirsutum) were found in NCBI and there have been no reports of transcriptome profiling analyzing anther development in Upland cotton, a major fiber crop in the word. METHODOLOGY/PRINCIPAL FINDING: Ninety-eight hundred and ninety-six high quality ESTs were sequenced from their 3′-ends and assembled into 6,643 unigenes from a normalized, full-length anther cDNA library of Upland cotton. Combined with previous sequenced anther-related ESTs, 12,244 unigenes were generated as the reference genes for digital gene expression (DGE) analysis. The DGE was conducted on anthers that were isolated at tetrad pollen (TTP), uninucleate pollen (UNP), binucleate pollen (BNP) and mature pollen (MTP) periods along with four other tissues, i.e., roots (RO), stems (ST), leaves (LV) and embryos (EB). Through transcriptome profiling analysis, we identified 1,165 genes that were enriched at certain anther development periods, and many of them were involved in starch and sucrose metabolism, pentose and glucuronate interconversion, flavonoid biosynthesis, and ascorbate and aldarate metabolism. CONCLUSIONS/SIGNIFICANCE: We first generated a normalized, full-length cDNA library from anthers and performed transcriptome profiling analysis of anther development in Upland cotton. From these results, 10,178 anther expressed genes were identified, among which 1,165 genes were stage-enriched in anthers. And many of these stage-enriched genes were involved in some important processes regulating anther development. Public Library of Science 2012-11-14 /pmc/articles/PMC3498337/ /pubmed/23155472 http://dx.doi.org/10.1371/journal.pone.0049244 Text en © 2012 Ma et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ma, Jianhui
Wei, Hengling
Song, Meizhen
Pang, Chaoyou
Liu, Ji
Wang, Long
Zhang, Jinfa
Fan, Shuli
Yu, Shuxun
Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
title Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
title_full Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
title_fullStr Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
title_full_unstemmed Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
title_short Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
title_sort transcriptome profiling analysis reveals that flavonoid and ascorbate-glutathione cycle are important during anther development in upland cotton
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498337/
https://www.ncbi.nlm.nih.gov/pubmed/23155472
http://dx.doi.org/10.1371/journal.pone.0049244
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