PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation

Abnormal amplification of centrosomes could lead to improper chromosome segregation and aneuploidy and is implicated in cancer development. Here, we demonstrate that Axin, a scaffolding protein in Wnt signaling, is phosphorylated by PLK1 during mitosis. Phosphorylation of Axin Ser-157 by PLK1 abolis...

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Detalles Bibliográficos
Autores principales: Ruan, Ka, Ye, Fan, Li, Chenyu, Liou, Yih-Cherng, Lin, Sheng-Cai, Lin, Shu-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498349/
https://www.ncbi.nlm.nih.gov/pubmed/23155463
http://dx.doi.org/10.1371/journal.pone.0049184
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author Ruan, Ka
Ye, Fan
Li, Chenyu
Liou, Yih-Cherng
Lin, Sheng-Cai
Lin, Shu-Yong
author_facet Ruan, Ka
Ye, Fan
Li, Chenyu
Liou, Yih-Cherng
Lin, Sheng-Cai
Lin, Shu-Yong
author_sort Ruan, Ka
collection PubMed
description Abnormal amplification of centrosomes could lead to improper chromosome segregation and aneuploidy and is implicated in cancer development. Here, we demonstrate that Axin, a scaffolding protein in Wnt signaling, is phosphorylated by PLK1 during mitosis. Phosphorylation of Axin Ser-157 by PLK1 abolished Axin association with γ-tubulin, while substitution of Ser-157 with alanine exhibited sustained interaction with γ-tubulin. In addition, overexpression of Axin-S157A significantly increased the number of cells with multi-centrosomes. These results suggest that the phosphorylation status of Axin, mediated by PLK1, dynamically regulates its association with γ-tubulin and centrosome formation and segregation.
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spelling pubmed-34983492012-11-15 PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation Ruan, Ka Ye, Fan Li, Chenyu Liou, Yih-Cherng Lin, Sheng-Cai Lin, Shu-Yong PLoS One Research Article Abnormal amplification of centrosomes could lead to improper chromosome segregation and aneuploidy and is implicated in cancer development. Here, we demonstrate that Axin, a scaffolding protein in Wnt signaling, is phosphorylated by PLK1 during mitosis. Phosphorylation of Axin Ser-157 by PLK1 abolished Axin association with γ-tubulin, while substitution of Ser-157 with alanine exhibited sustained interaction with γ-tubulin. In addition, overexpression of Axin-S157A significantly increased the number of cells with multi-centrosomes. These results suggest that the phosphorylation status of Axin, mediated by PLK1, dynamically regulates its association with γ-tubulin and centrosome formation and segregation. Public Library of Science 2012-11-14 /pmc/articles/PMC3498349/ /pubmed/23155463 http://dx.doi.org/10.1371/journal.pone.0049184 Text en © 2012 Ruan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ruan, Ka
Ye, Fan
Li, Chenyu
Liou, Yih-Cherng
Lin, Sheng-Cai
Lin, Shu-Yong
PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation
title PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation
title_full PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation
title_fullStr PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation
title_full_unstemmed PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation
title_short PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation
title_sort plk1 interacts and phosphorylates axin that is essential for proper centrosome formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498349/
https://www.ncbi.nlm.nih.gov/pubmed/23155463
http://dx.doi.org/10.1371/journal.pone.0049184
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