Cargando…
A fast, simple and robust protocol for growing crystals in the lipidic cubic phase
A simple and inexpensive protocol for producing crystals in the sticky and viscous mesophase used for membrane protein crystallization by the in meso method is described. It provides crystals that appear within 15–30 min of setup at 293 K. The protocol gives the experimenter a convenient way of gain...
Autores principales: | Aherne, Margaret, Lyons, Joseph A., Caffrey, Martin |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498651/ https://www.ncbi.nlm.nih.gov/pubmed/23162163 http://dx.doi.org/10.1107/S0021889812037880 |
Ejemplares similares
-
Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates
por: Barthel, Tatjana, et al.
Publicado: (2021) -
Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field
por: Magay, Elena, et al.
Publicado: (2012) -
3D-printed holders for in meso in situ fixed-target serial X-ray crystallography
por: Huang, Chia-Ying, et al.
Publicado: (2020) -
Control of the rate of evaporation in protein crystallization by the ‘microbatch under oil’ method
por: Brumshtein, Boris, et al.
Publicado: (2008) -
Experimental phasing for structure determination using membrane-protein crystals grown by the lipid cubic phase method
por: Li, Dianfan, et al.
Publicado: (2015)