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Uptake of radiolabeled GlcNAc into Saccharomyces cerevisiae via native hexose transporters and its in vivo incorporation into GPI precursors in cells expressing heterologous GlcNAc kinase

Yeast glycan biosynthetic pathways are commonly studied through metabolic incorporation of an exogenous radiolabeled compound into a target glycan. In Saccharomyces cerevisiae glycosylphosphatidylinositol (GPI) biosynthesis, [(3)H]inositol has been widely used to identify intermediates that accumula...

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Detalles Bibliográficos
Autores principales: Scarcelli, John J, Colussi, Paul A, Fabre, Anne-Lise, Boles, Eckhard, Orlean, Peter, Taron, Christopher H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498731/
https://www.ncbi.nlm.nih.gov/pubmed/22151002
http://dx.doi.org/10.1111/j.1567-1364.2011.00778.x