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Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures
Extremely low specific growth rates (below 0.01 h(−1)) represent a largely unexplored area of microbial physiology. In this study, anaerobic, glucose-limited retentostats were used to analyse physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to cultivation at near-z...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498732/ https://www.ncbi.nlm.nih.gov/pubmed/22093745 http://dx.doi.org/10.1111/j.1567-1364.2011.00750.x |
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author | Boender, Léonie GM Maris, Antonius JA Hulster, Erik AF Almering, Marinka JH Klei, Ida J Veenhuis, Marten Winde, Johannes H Pronk, Jack T Daran-Lapujade, Pascale |
author_facet | Boender, Léonie GM Maris, Antonius JA Hulster, Erik AF Almering, Marinka JH Klei, Ida J Veenhuis, Marten Winde, Johannes H Pronk, Jack T Daran-Lapujade, Pascale |
author_sort | Boender, Léonie GM |
collection | PubMed |
description | Extremely low specific growth rates (below 0.01 h(−1)) represent a largely unexplored area of microbial physiology. In this study, anaerobic, glucose-limited retentostats were used to analyse physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to cultivation at near-zero specific growth rates. While quiescence is typically investigated as a result of carbon starvation, cells in retentostat are fed by small, but continuous carbon and energy supply. Yeast cells cultivated near-zero specific growth rates, while metabolically active, exhibited characteristics previously associated with quiescence, including accumulation of storage polymers and an increased expression of genes involved in exit from the cell cycle into G(0). Unexpectedly, analysis of transcriptome data from retentostat and chemostat cultures showed, as specific growth rate was decreased, that quiescence-related transcriptional responses were already set in at specific growth rates above 0.025 h(−1). These observations stress the need for systematic dissection of physiological responses to slow growth, quiescence, ageing and starvation and indicate that controlled cultivation systems such as retentostats can contribute to this goal. Furthermore, cells in retentostat do not (or hardly) divide while remaining metabolically active, which emulates the physiological status of metazoan post-mitotic cells. We propose retentostat as a powerful cultivation tool to investigate chronological ageing-related processes. |
format | Online Article Text |
id | pubmed-3498732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-34987322012-11-15 Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures Boender, Léonie GM Maris, Antonius JA Hulster, Erik AF Almering, Marinka JH Klei, Ida J Veenhuis, Marten Winde, Johannes H Pronk, Jack T Daran-Lapujade, Pascale FEMS Yeast Res Research Articles Extremely low specific growth rates (below 0.01 h(−1)) represent a largely unexplored area of microbial physiology. In this study, anaerobic, glucose-limited retentostats were used to analyse physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to cultivation at near-zero specific growth rates. While quiescence is typically investigated as a result of carbon starvation, cells in retentostat are fed by small, but continuous carbon and energy supply. Yeast cells cultivated near-zero specific growth rates, while metabolically active, exhibited characteristics previously associated with quiescence, including accumulation of storage polymers and an increased expression of genes involved in exit from the cell cycle into G(0). Unexpectedly, analysis of transcriptome data from retentostat and chemostat cultures showed, as specific growth rate was decreased, that quiescence-related transcriptional responses were already set in at specific growth rates above 0.025 h(−1). These observations stress the need for systematic dissection of physiological responses to slow growth, quiescence, ageing and starvation and indicate that controlled cultivation systems such as retentostats can contribute to this goal. Furthermore, cells in retentostat do not (or hardly) divide while remaining metabolically active, which emulates the physiological status of metazoan post-mitotic cells. We propose retentostat as a powerful cultivation tool to investigate chronological ageing-related processes. Blackwell Publishing Ltd 2011-12 2011-09-26 /pmc/articles/PMC3498732/ /pubmed/22093745 http://dx.doi.org/10.1111/j.1567-1364.2011.00750.x Text en Copyright © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Research Articles Boender, Léonie GM Maris, Antonius JA Hulster, Erik AF Almering, Marinka JH Klei, Ida J Veenhuis, Marten Winde, Johannes H Pronk, Jack T Daran-Lapujade, Pascale Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures |
title | Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures |
title_full | Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures |
title_fullStr | Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures |
title_full_unstemmed | Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures |
title_short | Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures |
title_sort | cellular responses of saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498732/ https://www.ncbi.nlm.nih.gov/pubmed/22093745 http://dx.doi.org/10.1111/j.1567-1364.2011.00750.x |
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