Cargando…
Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction
Programmed ribosomal frameshifting is used in the expression of many virus genes and some cellular genes. In eukaryotic systems, the most well-characterized mechanism involves –1 tandem tRNA slippage on an X_XXY_YYZ motif. By contrast, the mechanisms involved in programmed +1 (or −2) slippage are mo...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498833/ https://www.ncbi.nlm.nih.gov/pubmed/23155484 http://dx.doi.org/10.1098/rsob.120109 |
_version_ | 1782249879859363840 |
---|---|
author | Firth, A. E. Jagger, B. W. Wise, H. M. Nelson, C. C. Parsawar, K. Wills, N. M. Napthine, S. Taubenberger, J. K. Digard, P. Atkins, J. F. |
author_facet | Firth, A. E. Jagger, B. W. Wise, H. M. Nelson, C. C. Parsawar, K. Wills, N. M. Napthine, S. Taubenberger, J. K. Digard, P. Atkins, J. F. |
author_sort | Firth, A. E. |
collection | PubMed |
description | Programmed ribosomal frameshifting is used in the expression of many virus genes and some cellular genes. In eukaryotic systems, the most well-characterized mechanism involves –1 tandem tRNA slippage on an X_XXY_YYZ motif. By contrast, the mechanisms involved in programmed +1 (or −2) slippage are more varied and often poorly characterized. Recently, a novel gene, PA-X, was discovered in influenza A virus and found to be expressed via a shift to the +1 reading frame. Here, we identify, by mass spectrometric analysis, both the site (UCC_UUU_CGU) and direction (+1) of the frameshifting that is involved in PA-X expression. Related sites are identified in other virus genes that have previously been proposed to be expressed via +1 frameshifting. As these viruses infect insects (chronic bee paralysis virus), plants (fijiviruses and amalgamaviruses) and vertebrates (influenza A virus), such motifs may form a new class of +1 frameshift-inducing sequences that are active in diverse eukaryotes. |
format | Online Article Text |
id | pubmed-3498833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-34988332012-11-15 Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction Firth, A. E. Jagger, B. W. Wise, H. M. Nelson, C. C. Parsawar, K. Wills, N. M. Napthine, S. Taubenberger, J. K. Digard, P. Atkins, J. F. Open Biol Research Programmed ribosomal frameshifting is used in the expression of many virus genes and some cellular genes. In eukaryotic systems, the most well-characterized mechanism involves –1 tandem tRNA slippage on an X_XXY_YYZ motif. By contrast, the mechanisms involved in programmed +1 (or −2) slippage are more varied and often poorly characterized. Recently, a novel gene, PA-X, was discovered in influenza A virus and found to be expressed via a shift to the +1 reading frame. Here, we identify, by mass spectrometric analysis, both the site (UCC_UUU_CGU) and direction (+1) of the frameshifting that is involved in PA-X expression. Related sites are identified in other virus genes that have previously been proposed to be expressed via +1 frameshifting. As these viruses infect insects (chronic bee paralysis virus), plants (fijiviruses and amalgamaviruses) and vertebrates (influenza A virus), such motifs may form a new class of +1 frameshift-inducing sequences that are active in diverse eukaryotes. The Royal Society 2012-10 /pmc/articles/PMC3498833/ /pubmed/23155484 http://dx.doi.org/10.1098/rsob.120109 Text en http://creativecommons.org/licenses/by/3.0/ © 2012 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research Firth, A. E. Jagger, B. W. Wise, H. M. Nelson, C. C. Parsawar, K. Wills, N. M. Napthine, S. Taubenberger, J. K. Digard, P. Atkins, J. F. Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction |
title | Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction |
title_full | Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction |
title_fullStr | Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction |
title_full_unstemmed | Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction |
title_short | Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction |
title_sort | ribosomal frameshifting used in influenza a virus expression occurs within the sequence ucc_uuu_cgu and is in the +1 direction |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498833/ https://www.ncbi.nlm.nih.gov/pubmed/23155484 http://dx.doi.org/10.1098/rsob.120109 |
work_keys_str_mv | AT firthae ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction AT jaggerbw ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction AT wisehm ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction AT nelsoncc ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction AT parsawark ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction AT willsnm ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction AT napthines ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction AT taubenbergerjk ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction AT digardp ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction AT atkinsjf ribosomalframeshiftingusedininfluenzaavirusexpressionoccurswithinthesequenceuccuuucguandisinthe1direction |