A Type III restriction–modification system in Mycoplasma mycoides subsp. capri

The sequenced genome of Mycoplasma mycoides subsp. capri revealed the presence of a Type III restriction–modification system (MmyCI). The methyltransferase (modification) subunit of MmyCI (M.MmyCI) was shown to recognize the sequence 5′-TGAG-3′ and methylate the adenine. The coding region of the met...

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Autores principales: Algire, Mikkel A., Montague, Michael G., Vashee, Sanjay, Lartigue, Carole, Merryman, Chuck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498834/
https://www.ncbi.nlm.nih.gov/pubmed/23155485
http://dx.doi.org/10.1098/rsob.120115
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author Algire, Mikkel A.
Montague, Michael G.
Vashee, Sanjay
Lartigue, Carole
Merryman, Chuck
author_facet Algire, Mikkel A.
Montague, Michael G.
Vashee, Sanjay
Lartigue, Carole
Merryman, Chuck
author_sort Algire, Mikkel A.
collection PubMed
description The sequenced genome of Mycoplasma mycoides subsp. capri revealed the presence of a Type III restriction–modification system (MmyCI). The methyltransferase (modification) subunit of MmyCI (M.MmyCI) was shown to recognize the sequence 5′-TGAG-3′ and methylate the adenine. The coding region of the methyltransferase gene contains 12 consecutive AG dinucleotide repeats that result in a translational termination at a TAA codon immediately beyond the repeat region. This strain does not have MmyCI activity. A clone was found with 10 AG repeats such that the gene is in frame, and this strain has MmyCI activity, suggesting that the expression of the MmyCI methyltransferase may be phase variable.
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spelling pubmed-34988342012-11-15 A Type III restriction–modification system in Mycoplasma mycoides subsp. capri Algire, Mikkel A. Montague, Michael G. Vashee, Sanjay Lartigue, Carole Merryman, Chuck Open Biol Research The sequenced genome of Mycoplasma mycoides subsp. capri revealed the presence of a Type III restriction–modification system (MmyCI). The methyltransferase (modification) subunit of MmyCI (M.MmyCI) was shown to recognize the sequence 5′-TGAG-3′ and methylate the adenine. The coding region of the methyltransferase gene contains 12 consecutive AG dinucleotide repeats that result in a translational termination at a TAA codon immediately beyond the repeat region. This strain does not have MmyCI activity. A clone was found with 10 AG repeats such that the gene is in frame, and this strain has MmyCI activity, suggesting that the expression of the MmyCI methyltransferase may be phase variable. The Royal Society 2012-10 /pmc/articles/PMC3498834/ /pubmed/23155485 http://dx.doi.org/10.1098/rsob.120115 Text en http://creativecommons.org/licenses/by/3.0/ © 2012 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research
Algire, Mikkel A.
Montague, Michael G.
Vashee, Sanjay
Lartigue, Carole
Merryman, Chuck
A Type III restriction–modification system in Mycoplasma mycoides subsp. capri
title A Type III restriction–modification system in Mycoplasma mycoides subsp. capri
title_full A Type III restriction–modification system in Mycoplasma mycoides subsp. capri
title_fullStr A Type III restriction–modification system in Mycoplasma mycoides subsp. capri
title_full_unstemmed A Type III restriction–modification system in Mycoplasma mycoides subsp. capri
title_short A Type III restriction–modification system in Mycoplasma mycoides subsp. capri
title_sort type iii restriction–modification system in mycoplasma mycoides subsp. capri
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498834/
https://www.ncbi.nlm.nih.gov/pubmed/23155485
http://dx.doi.org/10.1098/rsob.120115
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