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Chemotactic Migration of T Cells towards Dendritic Cells Promotes the Detection of Rare Antigens

In many immunological processes chemoattraction is thought to play a role in guiding cells to their sites of action. However, based on in vivo two-photon microscopy experiments in the absence of cognate antigen, T cell migration in lymph nodes (LNs) has been roughly described as a random walk. Altho...

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Autores principales: Vroomans, Renske M. A., Marée, Athanasius F. M., de Boer, Rob J., Beltman, Joost B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499258/
https://www.ncbi.nlm.nih.gov/pubmed/23166480
http://dx.doi.org/10.1371/journal.pcbi.1002763
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author Vroomans, Renske M. A.
Marée, Athanasius F. M.
de Boer, Rob J.
Beltman, Joost B.
author_facet Vroomans, Renske M. A.
Marée, Athanasius F. M.
de Boer, Rob J.
Beltman, Joost B.
author_sort Vroomans, Renske M. A.
collection PubMed
description In many immunological processes chemoattraction is thought to play a role in guiding cells to their sites of action. However, based on in vivo two-photon microscopy experiments in the absence of cognate antigen, T cell migration in lymph nodes (LNs) has been roughly described as a random walk. Although it has been shown that dendritic cells (DCs) carrying cognate antigen in some circumstances attract T cells chemotactically, it is currently still unclear whether chemoattraction of T cells towards DCs helps or hampers scanning. Chemoattraction towards DCs could on the one hand help T cells to rapidly find DCs. On the other hand, it could be deleterious if DCs become shielded by a multitude of attracted yet non-specific T cells. Results from a recent simulation study suggested that the deleterious effect dominates. We re-addressed the question whether T cell chemoattraction towards DCs is expected to promote or hamper the detection of rare antigens using the Cellular Potts Model, a formalism that allows for dynamic, flexible cellular shapes and cell migration. Our simulations show that chemoattraction of T cells enhances the DC scanning efficiency, leading to an increased probability that rare antigen-specific T cells find DCs carrying cognate antigen. Desensitization of T cells after contact with a DC further improves the scanning efficiency, yielding an almost threefold enhancement compared to random migration. Moreover, the chemotaxis-driven migration still roughly appears as a random walk, hence fine-tuned analysis of cell tracks will be required to detect chemotaxis within microscopy data.
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spelling pubmed-34992582012-11-19 Chemotactic Migration of T Cells towards Dendritic Cells Promotes the Detection of Rare Antigens Vroomans, Renske M. A. Marée, Athanasius F. M. de Boer, Rob J. Beltman, Joost B. PLoS Comput Biol Research Article In many immunological processes chemoattraction is thought to play a role in guiding cells to their sites of action. However, based on in vivo two-photon microscopy experiments in the absence of cognate antigen, T cell migration in lymph nodes (LNs) has been roughly described as a random walk. Although it has been shown that dendritic cells (DCs) carrying cognate antigen in some circumstances attract T cells chemotactically, it is currently still unclear whether chemoattraction of T cells towards DCs helps or hampers scanning. Chemoattraction towards DCs could on the one hand help T cells to rapidly find DCs. On the other hand, it could be deleterious if DCs become shielded by a multitude of attracted yet non-specific T cells. Results from a recent simulation study suggested that the deleterious effect dominates. We re-addressed the question whether T cell chemoattraction towards DCs is expected to promote or hamper the detection of rare antigens using the Cellular Potts Model, a formalism that allows for dynamic, flexible cellular shapes and cell migration. Our simulations show that chemoattraction of T cells enhances the DC scanning efficiency, leading to an increased probability that rare antigen-specific T cells find DCs carrying cognate antigen. Desensitization of T cells after contact with a DC further improves the scanning efficiency, yielding an almost threefold enhancement compared to random migration. Moreover, the chemotaxis-driven migration still roughly appears as a random walk, hence fine-tuned analysis of cell tracks will be required to detect chemotaxis within microscopy data. Public Library of Science 2012-11-15 /pmc/articles/PMC3499258/ /pubmed/23166480 http://dx.doi.org/10.1371/journal.pcbi.1002763 Text en © 2012 Vroomans et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Vroomans, Renske M. A.
Marée, Athanasius F. M.
de Boer, Rob J.
Beltman, Joost B.
Chemotactic Migration of T Cells towards Dendritic Cells Promotes the Detection of Rare Antigens
title Chemotactic Migration of T Cells towards Dendritic Cells Promotes the Detection of Rare Antigens
title_full Chemotactic Migration of T Cells towards Dendritic Cells Promotes the Detection of Rare Antigens
title_fullStr Chemotactic Migration of T Cells towards Dendritic Cells Promotes the Detection of Rare Antigens
title_full_unstemmed Chemotactic Migration of T Cells towards Dendritic Cells Promotes the Detection of Rare Antigens
title_short Chemotactic Migration of T Cells towards Dendritic Cells Promotes the Detection of Rare Antigens
title_sort chemotactic migration of t cells towards dendritic cells promotes the detection of rare antigens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499258/
https://www.ncbi.nlm.nih.gov/pubmed/23166480
http://dx.doi.org/10.1371/journal.pcbi.1002763
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