A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1

An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein β subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with...

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Autores principales: Kobayashi, Shio, Tsugama, Daisuke, Liu, Shenkui, Takano, Tetsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499536/
https://www.ncbi.nlm.nih.gov/pubmed/23166612
http://dx.doi.org/10.1371/journal.pone.0049207
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author Kobayashi, Shio
Tsugama, Daisuke
Liu, Shenkui
Takano, Tetsuo
author_facet Kobayashi, Shio
Tsugama, Daisuke
Liu, Shenkui
Takano, Tetsuo
author_sort Kobayashi, Shio
collection PubMed
description An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein β subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with AGB1 in the nuclei and the cytosol. The expression levels of PUB20 and its closest homolog, PUB21 were stable under many conditions. GUS driven by the PUB20 promoter was active in anthers, pollen, premature seeds and receptacles and GUS driven by the PUB21 promoter was active in anthers and funiculi. PUB20 was found to have autoubiquitination activity in vitro.
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spelling pubmed-34995362012-11-19 A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1 Kobayashi, Shio Tsugama, Daisuke Liu, Shenkui Takano, Tetsuo PLoS One Research Article An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein β subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with AGB1 in the nuclei and the cytosol. The expression levels of PUB20 and its closest homolog, PUB21 were stable under many conditions. GUS driven by the PUB20 promoter was active in anthers, pollen, premature seeds and receptacles and GUS driven by the PUB21 promoter was active in anthers and funiculi. PUB20 was found to have autoubiquitination activity in vitro. Public Library of Science 2012-11-15 /pmc/articles/PMC3499536/ /pubmed/23166612 http://dx.doi.org/10.1371/journal.pone.0049207 Text en © 2012 Kobayashi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kobayashi, Shio
Tsugama, Daisuke
Liu, Shenkui
Takano, Tetsuo
A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1
title A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1
title_full A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1
title_fullStr A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1
title_full_unstemmed A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1
title_short A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1
title_sort u-box e3 ubiquitin ligase, pub20, interacts with the arabidopsis g-protein β subunit, agb1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499536/
https://www.ncbi.nlm.nih.gov/pubmed/23166612
http://dx.doi.org/10.1371/journal.pone.0049207
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