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A Novel Strain D5 Isolated from Acacia confusa
We isolated a novel strain D5 from nodules of Acacia confusa. Under strict sterile conditions the strain could successfully nodulate Acacia confusa, A. crassicarpa and A. mangium, with nitrogenase activity ranging from 18.90 to 19.86 nmol·g(−1)·min(−1). In the phylogenetic tree based on a complete 1...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499541/ https://www.ncbi.nlm.nih.gov/pubmed/23166618 http://dx.doi.org/10.1371/journal.pone.0049236 |
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author | Huang, Baoling Lv, Chengqun Zhao, Yili Huang, Rong |
author_facet | Huang, Baoling Lv, Chengqun Zhao, Yili Huang, Rong |
author_sort | Huang, Baoling |
collection | PubMed |
description | We isolated a novel strain D5 from nodules of Acacia confusa. Under strict sterile conditions the strain could successfully nodulate Acacia confusa, A. crassicarpa and A. mangium, with nitrogenase activity ranging from 18.90 to 19.86 nmol·g(−1)·min(−1). In the phylogenetic tree based on a complete 16S rRNA gene sequence, the sequence of strain D5 shared 99% homology with that of four species of genus Pseudomonas. The 685 bp nodA fragment amplified from strain D5 shared 95% homology with the nodA sequence of 9 species of genus Bradyrhizobium, with a genetic distance of 0.01682. The 740 bp nifH gene fragment was amplified from strain D5. This strain D5 nifH gene and Bradyrhizobium spp. formed a branch, showing 98% homology and a genetic distance of 0. The homology between this branch and the Bradyrhizobium spp. DG in another branch was 99%, with a genetic distance of 0.007906. These results indicate that this strain D5 is a new type of nitrogen-fixing bacterium. |
format | Online Article Text |
id | pubmed-3499541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34995412012-11-19 A Novel Strain D5 Isolated from Acacia confusa Huang, Baoling Lv, Chengqun Zhao, Yili Huang, Rong PLoS One Research Article We isolated a novel strain D5 from nodules of Acacia confusa. Under strict sterile conditions the strain could successfully nodulate Acacia confusa, A. crassicarpa and A. mangium, with nitrogenase activity ranging from 18.90 to 19.86 nmol·g(−1)·min(−1). In the phylogenetic tree based on a complete 16S rRNA gene sequence, the sequence of strain D5 shared 99% homology with that of four species of genus Pseudomonas. The 685 bp nodA fragment amplified from strain D5 shared 95% homology with the nodA sequence of 9 species of genus Bradyrhizobium, with a genetic distance of 0.01682. The 740 bp nifH gene fragment was amplified from strain D5. This strain D5 nifH gene and Bradyrhizobium spp. formed a branch, showing 98% homology and a genetic distance of 0. The homology between this branch and the Bradyrhizobium spp. DG in another branch was 99%, with a genetic distance of 0.007906. These results indicate that this strain D5 is a new type of nitrogen-fixing bacterium. Public Library of Science 2012-11-15 /pmc/articles/PMC3499541/ /pubmed/23166618 http://dx.doi.org/10.1371/journal.pone.0049236 Text en © 2012 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Huang, Baoling Lv, Chengqun Zhao, Yili Huang, Rong A Novel Strain D5 Isolated from Acacia confusa |
title | A Novel Strain D5 Isolated from Acacia confusa
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title_full | A Novel Strain D5 Isolated from Acacia confusa
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title_fullStr | A Novel Strain D5 Isolated from Acacia confusa
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title_full_unstemmed | A Novel Strain D5 Isolated from Acacia confusa
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title_short | A Novel Strain D5 Isolated from Acacia confusa
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title_sort | novel strain d5 isolated from acacia confusa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499541/ https://www.ncbi.nlm.nih.gov/pubmed/23166618 http://dx.doi.org/10.1371/journal.pone.0049236 |
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