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A Novel Strain D5 Isolated from Acacia confusa

We isolated a novel strain D5 from nodules of Acacia confusa. Under strict sterile conditions the strain could successfully nodulate Acacia confusa, A. crassicarpa and A. mangium, with nitrogenase activity ranging from 18.90 to 19.86 nmol·g(−1)·min(−1). In the phylogenetic tree based on a complete 1...

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Detalles Bibliográficos
Autores principales: Huang, Baoling, Lv, Chengqun, Zhao, Yili, Huang, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499541/
https://www.ncbi.nlm.nih.gov/pubmed/23166618
http://dx.doi.org/10.1371/journal.pone.0049236
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author Huang, Baoling
Lv, Chengqun
Zhao, Yili
Huang, Rong
author_facet Huang, Baoling
Lv, Chengqun
Zhao, Yili
Huang, Rong
author_sort Huang, Baoling
collection PubMed
description We isolated a novel strain D5 from nodules of Acacia confusa. Under strict sterile conditions the strain could successfully nodulate Acacia confusa, A. crassicarpa and A. mangium, with nitrogenase activity ranging from 18.90 to 19.86 nmol·g(−1)·min(−1). In the phylogenetic tree based on a complete 16S rRNA gene sequence, the sequence of strain D5 shared 99% homology with that of four species of genus Pseudomonas. The 685 bp nodA fragment amplified from strain D5 shared 95% homology with the nodA sequence of 9 species of genus Bradyrhizobium, with a genetic distance of 0.01682. The 740 bp nifH gene fragment was amplified from strain D5. This strain D5 nifH gene and Bradyrhizobium spp. formed a branch, showing 98% homology and a genetic distance of 0. The homology between this branch and the Bradyrhizobium spp. DG in another branch was 99%, with a genetic distance of 0.007906. These results indicate that this strain D5 is a new type of nitrogen-fixing bacterium.
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spelling pubmed-34995412012-11-19 A Novel Strain D5 Isolated from Acacia confusa Huang, Baoling Lv, Chengqun Zhao, Yili Huang, Rong PLoS One Research Article We isolated a novel strain D5 from nodules of Acacia confusa. Under strict sterile conditions the strain could successfully nodulate Acacia confusa, A. crassicarpa and A. mangium, with nitrogenase activity ranging from 18.90 to 19.86 nmol·g(−1)·min(−1). In the phylogenetic tree based on a complete 16S rRNA gene sequence, the sequence of strain D5 shared 99% homology with that of four species of genus Pseudomonas. The 685 bp nodA fragment amplified from strain D5 shared 95% homology with the nodA sequence of 9 species of genus Bradyrhizobium, with a genetic distance of 0.01682. The 740 bp nifH gene fragment was amplified from strain D5. This strain D5 nifH gene and Bradyrhizobium spp. formed a branch, showing 98% homology and a genetic distance of 0. The homology between this branch and the Bradyrhizobium spp. DG in another branch was 99%, with a genetic distance of 0.007906. These results indicate that this strain D5 is a new type of nitrogen-fixing bacterium. Public Library of Science 2012-11-15 /pmc/articles/PMC3499541/ /pubmed/23166618 http://dx.doi.org/10.1371/journal.pone.0049236 Text en © 2012 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Huang, Baoling
Lv, Chengqun
Zhao, Yili
Huang, Rong
A Novel Strain D5 Isolated from Acacia confusa
title A Novel Strain D5 Isolated from Acacia confusa
title_full A Novel Strain D5 Isolated from Acacia confusa
title_fullStr A Novel Strain D5 Isolated from Acacia confusa
title_full_unstemmed A Novel Strain D5 Isolated from Acacia confusa
title_short A Novel Strain D5 Isolated from Acacia confusa
title_sort novel strain d5 isolated from acacia confusa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499541/
https://www.ncbi.nlm.nih.gov/pubmed/23166618
http://dx.doi.org/10.1371/journal.pone.0049236
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