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Phylogenetic Analysis and Comparative Genomics of Purine Riboswitch Distribution in Prokaryotes

Riboswitches are regulatory RNA that control gene expression by undergoing conformational changes on ligand binding. Using phylogenetic analysis and comparative genomics we have been able to identify the class of genes/operons regulated by the purine riboswitch and obtain a high-resolution map of pu...

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Detalles Bibliográficos
Autores principales: Singh, Payal, Sengupta, Supratim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499989/
https://www.ncbi.nlm.nih.gov/pubmed/23170063
http://dx.doi.org/10.4137/EBO.S10048
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author Singh, Payal
Sengupta, Supratim
author_facet Singh, Payal
Sengupta, Supratim
author_sort Singh, Payal
collection PubMed
description Riboswitches are regulatory RNA that control gene expression by undergoing conformational changes on ligand binding. Using phylogenetic analysis and comparative genomics we have been able to identify the class of genes/operons regulated by the purine riboswitch and obtain a high-resolution map of purine riboswitch distribution across all bacterial groups. In the process, we are able to explain the absence of purine riboswitches upstream to specific genes in certain genomes. We also identify the point of origin of various purine riboswitches and argue that not all purine riboswitches are of primordial origin, and that some purine riboswitches must have originated after the divergence of certain Firmicute orders in the course of evolution. Our study also reveals the role of horizontal transfer events in accounting for the presence of purine riboswitches in some gammaproteobacterial species. Our work provides significant insights into the origin, distribution and regulatory role of purine riboswitches in prokaryotes.
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spelling pubmed-34999892012-11-20 Phylogenetic Analysis and Comparative Genomics of Purine Riboswitch Distribution in Prokaryotes Singh, Payal Sengupta, Supratim Evol Bioinform Online Original Research Riboswitches are regulatory RNA that control gene expression by undergoing conformational changes on ligand binding. Using phylogenetic analysis and comparative genomics we have been able to identify the class of genes/operons regulated by the purine riboswitch and obtain a high-resolution map of purine riboswitch distribution across all bacterial groups. In the process, we are able to explain the absence of purine riboswitches upstream to specific genes in certain genomes. We also identify the point of origin of various purine riboswitches and argue that not all purine riboswitches are of primordial origin, and that some purine riboswitches must have originated after the divergence of certain Firmicute orders in the course of evolution. Our study also reveals the role of horizontal transfer events in accounting for the presence of purine riboswitches in some gammaproteobacterial species. Our work provides significant insights into the origin, distribution and regulatory role of purine riboswitches in prokaryotes. Libertas Academica 2012-11-06 /pmc/articles/PMC3499989/ /pubmed/23170063 http://dx.doi.org/10.4137/EBO.S10048 Text en © 2012 the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Original Research
Singh, Payal
Sengupta, Supratim
Phylogenetic Analysis and Comparative Genomics of Purine Riboswitch Distribution in Prokaryotes
title Phylogenetic Analysis and Comparative Genomics of Purine Riboswitch Distribution in Prokaryotes
title_full Phylogenetic Analysis and Comparative Genomics of Purine Riboswitch Distribution in Prokaryotes
title_fullStr Phylogenetic Analysis and Comparative Genomics of Purine Riboswitch Distribution in Prokaryotes
title_full_unstemmed Phylogenetic Analysis and Comparative Genomics of Purine Riboswitch Distribution in Prokaryotes
title_short Phylogenetic Analysis and Comparative Genomics of Purine Riboswitch Distribution in Prokaryotes
title_sort phylogenetic analysis and comparative genomics of purine riboswitch distribution in prokaryotes
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499989/
https://www.ncbi.nlm.nih.gov/pubmed/23170063
http://dx.doi.org/10.4137/EBO.S10048
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