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Comparative Analysis of Genome Sequences Covering the Seven Cronobacter Species

BACKGROUND: Species of Cronobacter are widespread in the environment and are occasional food-borne pathogens associated with serious neonatal diseases, including bacteraemia, meningitis, and necrotising enterocolitis. The genus is composed of seven species: C. sakazakii, C. malonaticus, C. turicensi...

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Autores principales: Joseph, Susan, Desai, Prerak, Ji, Yongmei, Cummings, Craig A., Shih, Rita, Degoricija, Lovorka, Rico, Alain, Brzoska, Pius, Hamby, Stephen E., Masood, Naqash, Hariri, Sumyya, Sonbol, Hana, Chuzhanova, Nadia, McClelland, Michael, Furtado, Manohar R., Forsythe, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3500316/
https://www.ncbi.nlm.nih.gov/pubmed/23166675
http://dx.doi.org/10.1371/journal.pone.0049455
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author Joseph, Susan
Desai, Prerak
Ji, Yongmei
Cummings, Craig A.
Shih, Rita
Degoricija, Lovorka
Rico, Alain
Brzoska, Pius
Hamby, Stephen E.
Masood, Naqash
Hariri, Sumyya
Sonbol, Hana
Chuzhanova, Nadia
McClelland, Michael
Furtado, Manohar R.
Forsythe, Stephen J.
author_facet Joseph, Susan
Desai, Prerak
Ji, Yongmei
Cummings, Craig A.
Shih, Rita
Degoricija, Lovorka
Rico, Alain
Brzoska, Pius
Hamby, Stephen E.
Masood, Naqash
Hariri, Sumyya
Sonbol, Hana
Chuzhanova, Nadia
McClelland, Michael
Furtado, Manohar R.
Forsythe, Stephen J.
author_sort Joseph, Susan
collection PubMed
description BACKGROUND: Species of Cronobacter are widespread in the environment and are occasional food-borne pathogens associated with serious neonatal diseases, including bacteraemia, meningitis, and necrotising enterocolitis. The genus is composed of seven species: C. sakazakii, C. malonaticus, C. turicensis, C. dublinensis, C. muytjensii, C. universalis, and C. condimenti. Clinical cases are associated with three species, C. malonaticus, C. turicensis and, in particular, with C. sakazakii multilocus sequence type 4. Thus, it is plausible that virulence determinants have evolved in certain lineages. METHODOLOGY/PRINCIPAL FINDINGS: We generated high quality sequence drafts for eleven Cronobacter genomes representing the seven Cronobacter species, including an ST4 strain of C. sakazakii. Comparative analysis of these genomes together with the two publicly available genomes revealed Cronobacter has over 6,000 genes in one or more strains and over 2,000 genes shared by all Cronobacter. Considerable variation in the presence of traits such as type six secretion systems, metal resistance (tellurite, copper and silver), and adhesins were found. C. sakazakii is unique in the Cronobacter genus in encoding genes enabling the utilization of exogenous sialic acid which may have clinical significance. The C. sakazakii ST4 strain 701 contained additional genes as compared to other C. sakazakii but none of them were known specific virulence-related genes. CONCLUSIONS/SIGNIFICANCE: Genome comparison revealed that pair-wise DNA sequence identity varies between 89 and 97% in the seven Cronobacter species, and also suggested various degrees of divergence. Sets of universal core genes and accessory genes unique to each strain were identified. These gene sequences can be used for designing genus/species specific detection assays. Genes encoding adhesins, T6SS, and metal resistance genes as well as prophages are found in only subsets of genomes and have contributed considerably to the variation of genomic content. Differences in gene content likely contribute to differences in the clinical and environmental distribution of species and sequence types.
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spelling pubmed-35003162012-11-19 Comparative Analysis of Genome Sequences Covering the Seven Cronobacter Species Joseph, Susan Desai, Prerak Ji, Yongmei Cummings, Craig A. Shih, Rita Degoricija, Lovorka Rico, Alain Brzoska, Pius Hamby, Stephen E. Masood, Naqash Hariri, Sumyya Sonbol, Hana Chuzhanova, Nadia McClelland, Michael Furtado, Manohar R. Forsythe, Stephen J. PLoS One Research Article BACKGROUND: Species of Cronobacter are widespread in the environment and are occasional food-borne pathogens associated with serious neonatal diseases, including bacteraemia, meningitis, and necrotising enterocolitis. The genus is composed of seven species: C. sakazakii, C. malonaticus, C. turicensis, C. dublinensis, C. muytjensii, C. universalis, and C. condimenti. Clinical cases are associated with three species, C. malonaticus, C. turicensis and, in particular, with C. sakazakii multilocus sequence type 4. Thus, it is plausible that virulence determinants have evolved in certain lineages. METHODOLOGY/PRINCIPAL FINDINGS: We generated high quality sequence drafts for eleven Cronobacter genomes representing the seven Cronobacter species, including an ST4 strain of C. sakazakii. Comparative analysis of these genomes together with the two publicly available genomes revealed Cronobacter has over 6,000 genes in one or more strains and over 2,000 genes shared by all Cronobacter. Considerable variation in the presence of traits such as type six secretion systems, metal resistance (tellurite, copper and silver), and adhesins were found. C. sakazakii is unique in the Cronobacter genus in encoding genes enabling the utilization of exogenous sialic acid which may have clinical significance. The C. sakazakii ST4 strain 701 contained additional genes as compared to other C. sakazakii but none of them were known specific virulence-related genes. CONCLUSIONS/SIGNIFICANCE: Genome comparison revealed that pair-wise DNA sequence identity varies between 89 and 97% in the seven Cronobacter species, and also suggested various degrees of divergence. Sets of universal core genes and accessory genes unique to each strain were identified. These gene sequences can be used for designing genus/species specific detection assays. Genes encoding adhesins, T6SS, and metal resistance genes as well as prophages are found in only subsets of genomes and have contributed considerably to the variation of genomic content. Differences in gene content likely contribute to differences in the clinical and environmental distribution of species and sequence types. Public Library of Science 2012-11-16 /pmc/articles/PMC3500316/ /pubmed/23166675 http://dx.doi.org/10.1371/journal.pone.0049455 Text en © 2012 Joseph et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Joseph, Susan
Desai, Prerak
Ji, Yongmei
Cummings, Craig A.
Shih, Rita
Degoricija, Lovorka
Rico, Alain
Brzoska, Pius
Hamby, Stephen E.
Masood, Naqash
Hariri, Sumyya
Sonbol, Hana
Chuzhanova, Nadia
McClelland, Michael
Furtado, Manohar R.
Forsythe, Stephen J.
Comparative Analysis of Genome Sequences Covering the Seven Cronobacter Species
title Comparative Analysis of Genome Sequences Covering the Seven Cronobacter Species
title_full Comparative Analysis of Genome Sequences Covering the Seven Cronobacter Species
title_fullStr Comparative Analysis of Genome Sequences Covering the Seven Cronobacter Species
title_full_unstemmed Comparative Analysis of Genome Sequences Covering the Seven Cronobacter Species
title_short Comparative Analysis of Genome Sequences Covering the Seven Cronobacter Species
title_sort comparative analysis of genome sequences covering the seven cronobacter species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3500316/
https://www.ncbi.nlm.nih.gov/pubmed/23166675
http://dx.doi.org/10.1371/journal.pone.0049455
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