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Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an X-ray fluorescence microscopy study

In the present paper, X-ray fluorescence microscopy was applied to follow the processes occurring in rat hippocampal formation during the post-seizure period. In the study, one of the status epilepticus animal models of epilepsy was used, namely the model of temporal lobe epilepsy with pilocarpine-i...

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Autores principales: Chwiej, J., Kutorasinska, J., Janeczko, K., Gzielo-Jurek, K., Uram, L., Appel, K., Simon, R., Setkowicz, Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501183/
https://www.ncbi.nlm.nih.gov/pubmed/23052869
http://dx.doi.org/10.1007/s00216-012-6425-5
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author Chwiej, J.
Kutorasinska, J.
Janeczko, K.
Gzielo-Jurek, K.
Uram, L.
Appel, K.
Simon, R.
Setkowicz, Z.
author_facet Chwiej, J.
Kutorasinska, J.
Janeczko, K.
Gzielo-Jurek, K.
Uram, L.
Appel, K.
Simon, R.
Setkowicz, Z.
author_sort Chwiej, J.
collection PubMed
description In the present paper, X-ray fluorescence microscopy was applied to follow the processes occurring in rat hippocampal formation during the post-seizure period. In the study, one of the status epilepticus animal models of epilepsy was used, namely the model of temporal lobe epilepsy with pilocarpine-induced seizures. In order to analyze the dynamics of seizure-induced elemental changes, the samples taken from seizure-experiencing animals 3 h and 1, 4, and 7 days after proconvulsive agent administration were analyzed. The obtained results confirmed the utility of X-ray fluorescence microscopy in the research of mechanisms involved in the pathogenesis and progress of epilepsy. The topographic and quantitative elemental analysis of hippocampal formations from different periods of epileptogenesis showed that excitotoxicity, mossy fibers sprouting, and iron-induced oxidative stress may be the processes responsible for seizure-induced neurodegenerative changes and spontaneous recurrent seizures occurring in the chronic phase of the pilocarpine model. The analysis of correlations between the recorded elemental anomalies and quantitative parameters describing animal behavior in the acute period of pilocarpine-induced status epilepticus showed that the areal densities of selected elements measured in the latent period strongly depend on the progress of the acute phase. Especially important seem to be the observations done for Ca and Zn levels which suggest that the intensity of the pathological processes such as excitotoxicity and mossy fibers sprouting depend on the total time of seizure activity. These results as well as dependencies found between the levels of S, K, and Cu and the intensity of maximal seizures clearly confirm how important it is to control the duration and intensity of seizures in clinical practice.
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spelling pubmed-35011832012-11-26 Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an X-ray fluorescence microscopy study Chwiej, J. Kutorasinska, J. Janeczko, K. Gzielo-Jurek, K. Uram, L. Appel, K. Simon, R. Setkowicz, Z. Anal Bioanal Chem Original Paper In the present paper, X-ray fluorescence microscopy was applied to follow the processes occurring in rat hippocampal formation during the post-seizure period. In the study, one of the status epilepticus animal models of epilepsy was used, namely the model of temporal lobe epilepsy with pilocarpine-induced seizures. In order to analyze the dynamics of seizure-induced elemental changes, the samples taken from seizure-experiencing animals 3 h and 1, 4, and 7 days after proconvulsive agent administration were analyzed. The obtained results confirmed the utility of X-ray fluorescence microscopy in the research of mechanisms involved in the pathogenesis and progress of epilepsy. The topographic and quantitative elemental analysis of hippocampal formations from different periods of epileptogenesis showed that excitotoxicity, mossy fibers sprouting, and iron-induced oxidative stress may be the processes responsible for seizure-induced neurodegenerative changes and spontaneous recurrent seizures occurring in the chronic phase of the pilocarpine model. The analysis of correlations between the recorded elemental anomalies and quantitative parameters describing animal behavior in the acute period of pilocarpine-induced status epilepticus showed that the areal densities of selected elements measured in the latent period strongly depend on the progress of the acute phase. Especially important seem to be the observations done for Ca and Zn levels which suggest that the intensity of the pathological processes such as excitotoxicity and mossy fibers sprouting depend on the total time of seizure activity. These results as well as dependencies found between the levels of S, K, and Cu and the intensity of maximal seizures clearly confirm how important it is to control the duration and intensity of seizures in clinical practice. Springer-Verlag 2012-10-04 2012 /pmc/articles/PMC3501183/ /pubmed/23052869 http://dx.doi.org/10.1007/s00216-012-6425-5 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Chwiej, J.
Kutorasinska, J.
Janeczko, K.
Gzielo-Jurek, K.
Uram, L.
Appel, K.
Simon, R.
Setkowicz, Z.
Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an X-ray fluorescence microscopy study
title Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an X-ray fluorescence microscopy study
title_full Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an X-ray fluorescence microscopy study
title_fullStr Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an X-ray fluorescence microscopy study
title_full_unstemmed Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an X-ray fluorescence microscopy study
title_short Progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an X-ray fluorescence microscopy study
title_sort progress of elemental anomalies of hippocampal formation in the pilocarpine model of temporal lobe epilepsy—an x-ray fluorescence microscopy study
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501183/
https://www.ncbi.nlm.nih.gov/pubmed/23052869
http://dx.doi.org/10.1007/s00216-012-6425-5
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