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Refractive index measurement of acute rat brain tissue slices using optical coherence tomography
An optical coherence tomography (OCT) system employing a microelectromechanical system (MEMS) mirror was used to measure the refractive index (RI) of anatomically different regions in acute brain tissue slices, in which viability was maintained. RI was measured in white-matter and grey-matter region...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501791/ https://www.ncbi.nlm.nih.gov/pubmed/22274454 http://dx.doi.org/10.1364/OE.20.001084 |
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author | Sun, Jingjing Lee, Sung Jin Wu, Lei Sarntinoranont, Malisa Xie, Huikai |
author_facet | Sun, Jingjing Lee, Sung Jin Wu, Lei Sarntinoranont, Malisa Xie, Huikai |
author_sort | Sun, Jingjing |
collection | PubMed |
description | An optical coherence tomography (OCT) system employing a microelectromechanical system (MEMS) mirror was used to measure the refractive index (RI) of anatomically different regions in acute brain tissue slices, in which viability was maintained. RI was measured in white-matter and grey-matter regions, including the cerebral cortex, putamen, hippocampus, thalamus and corpus callosum. The RI in the corpus callosum was found to be ~4% higher than the RIs in other regions. Changes in RI with tissue deformation were also measured in the cerebral cortex and corpus callosum under uniform compression (20-80% strain). For 80% strain, measured RIs increased nonlinearly by up to 70% and 90% in the cerebral cortex and corpus callosum respectively. Knowledge of RI in heterogeneous tissues can be used to correct distorted optical images caused by RI variations between different regions. Also deformation-dependent changes in RI can be applied to OCT elastography or to mechanical tests based on optical imaging such as indentation tests. |
format | Online Article Text |
id | pubmed-3501791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-35017912013-01-04 Refractive index measurement of acute rat brain tissue slices using optical coherence tomography Sun, Jingjing Lee, Sung Jin Wu, Lei Sarntinoranont, Malisa Xie, Huikai Opt Express Research-Article An optical coherence tomography (OCT) system employing a microelectromechanical system (MEMS) mirror was used to measure the refractive index (RI) of anatomically different regions in acute brain tissue slices, in which viability was maintained. RI was measured in white-matter and grey-matter regions, including the cerebral cortex, putamen, hippocampus, thalamus and corpus callosum. The RI in the corpus callosum was found to be ~4% higher than the RIs in other regions. Changes in RI with tissue deformation were also measured in the cerebral cortex and corpus callosum under uniform compression (20-80% strain). For 80% strain, measured RIs increased nonlinearly by up to 70% and 90% in the cerebral cortex and corpus callosum respectively. Knowledge of RI in heterogeneous tissues can be used to correct distorted optical images caused by RI variations between different regions. Also deformation-dependent changes in RI can be applied to OCT elastography or to mechanical tests based on optical imaging such as indentation tests. Optical Society of America 2012-01-04 /pmc/articles/PMC3501791/ /pubmed/22274454 http://dx.doi.org/10.1364/OE.20.001084 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Research-Article Sun, Jingjing Lee, Sung Jin Wu, Lei Sarntinoranont, Malisa Xie, Huikai Refractive index measurement of acute rat brain tissue slices using optical coherence tomography |
title | Refractive index measurement of acute rat brain tissue slices using optical coherence tomography |
title_full | Refractive index measurement of acute rat brain tissue slices using optical coherence tomography |
title_fullStr | Refractive index measurement of acute rat brain tissue slices using optical coherence tomography |
title_full_unstemmed | Refractive index measurement of acute rat brain tissue slices using optical coherence tomography |
title_short | Refractive index measurement of acute rat brain tissue slices using optical coherence tomography |
title_sort | refractive index measurement of acute rat brain tissue slices using optical coherence tomography |
topic | Research-Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501791/ https://www.ncbi.nlm.nih.gov/pubmed/22274454 http://dx.doi.org/10.1364/OE.20.001084 |
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