Cargando…

Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid

Trinitrobenzene sulfonic acid (TNBS)-induced colitis is one of the most common methods for studying inflammatory bowel disease in animal models. Several factors may, however, affect its reproducibility, rate of animal mortality, and macroscopic and histopathological outcomes. Our aim was to validate...

Descripción completa

Detalles Bibliográficos
Autores principales: Motavallian-Naeini, A., Andalib, S., Rabbani, M., Mahzouni, P., Afsharipour, M., Minaiyan, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501925/
https://www.ncbi.nlm.nih.gov/pubmed/23181094
_version_ 1782250242874277888
author Motavallian-Naeini, A.
Andalib, S.
Rabbani, M.
Mahzouni, P.
Afsharipour, M.
Minaiyan, M.
author_facet Motavallian-Naeini, A.
Andalib, S.
Rabbani, M.
Mahzouni, P.
Afsharipour, M.
Minaiyan, M.
author_sort Motavallian-Naeini, A.
collection PubMed
description Trinitrobenzene sulfonic acid (TNBS)-induced colitis is one of the most common methods for studying inflammatory bowel disease in animal models. Several factors may, however, affect its reproducibility, rate of animal mortality, and macroscopic and histopathological outcomes. Our aim was to validate the main contributing factors to this method and compare the effects of different reference drugs upon remission of resultant colon injuries. TNBS was dissolved in 0.25 ml of ethanol (50% v/v) and instilled (25, 50, 100 and 150 mg/kg) intracolonically to the male Wistar rats. After determination of optimum dose of TNBS in male rats and assessment of this dose in female rats, they were treated with reference drugs including dexamethasone [1 mg/kg, intraperitoneally (i.p.) and 2 mg/kg, orally (p.o.)], Asacol (mesalazine, 100 mg/kg, p.o.; 150 mg/kg, enema) and hydrocortisone acetate (20 mg/kg, i.p.; 20 mg/kg, enema) which started 2 h after colitis induction and continued daily for 6 consecutive days. Thereafter, macroscopic and microscopic parameters and clinical features were assessed and compared in different groups. We found that the optimum dose of TNBS for the reproducibility of colonic damage with the least mortality rate was 50 mg/kg. Amongst studied reference drugs, hydrocortisone acetate (i.p.), dexamethasone (i.p. and p.o.) and Asacol (p.o.) significantly diminished the severity of macroscopic and microscopic injuries and could be considered effective for experimental colitis studies in rats . Our findings suggest that optimization of TNBS dose is essential for induction of colitis under the laboratory conditions; and gender exerts no impact upon macroscopic and histological characteristics of TNBS-induced colitis in rats. Furthermore, the enema forms of hydrocortisone and Asacol are not appropriate reference drugs.
format Online
Article
Text
id pubmed-3501925
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-35019252012-11-23 Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid Motavallian-Naeini, A. Andalib, S. Rabbani, M. Mahzouni, P. Afsharipour, M. Minaiyan, M. Res Pharm Sci Original Article Trinitrobenzene sulfonic acid (TNBS)-induced colitis is one of the most common methods for studying inflammatory bowel disease in animal models. Several factors may, however, affect its reproducibility, rate of animal mortality, and macroscopic and histopathological outcomes. Our aim was to validate the main contributing factors to this method and compare the effects of different reference drugs upon remission of resultant colon injuries. TNBS was dissolved in 0.25 ml of ethanol (50% v/v) and instilled (25, 50, 100 and 150 mg/kg) intracolonically to the male Wistar rats. After determination of optimum dose of TNBS in male rats and assessment of this dose in female rats, they were treated with reference drugs including dexamethasone [1 mg/kg, intraperitoneally (i.p.) and 2 mg/kg, orally (p.o.)], Asacol (mesalazine, 100 mg/kg, p.o.; 150 mg/kg, enema) and hydrocortisone acetate (20 mg/kg, i.p.; 20 mg/kg, enema) which started 2 h after colitis induction and continued daily for 6 consecutive days. Thereafter, macroscopic and microscopic parameters and clinical features were assessed and compared in different groups. We found that the optimum dose of TNBS for the reproducibility of colonic damage with the least mortality rate was 50 mg/kg. Amongst studied reference drugs, hydrocortisone acetate (i.p.), dexamethasone (i.p. and p.o.) and Asacol (p.o.) significantly diminished the severity of macroscopic and microscopic injuries and could be considered effective for experimental colitis studies in rats . Our findings suggest that optimization of TNBS dose is essential for induction of colitis under the laboratory conditions; and gender exerts no impact upon macroscopic and histological characteristics of TNBS-induced colitis in rats. Furthermore, the enema forms of hydrocortisone and Asacol are not appropriate reference drugs. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3501925/ /pubmed/23181094 Text en Copyright: © Journal of Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Motavallian-Naeini, A.
Andalib, S.
Rabbani, M.
Mahzouni, P.
Afsharipour, M.
Minaiyan, M.
Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid
title Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid
title_full Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid
title_fullStr Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid
title_full_unstemmed Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid
title_short Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid
title_sort validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501925/
https://www.ncbi.nlm.nih.gov/pubmed/23181094
work_keys_str_mv AT motavalliannaeinia validationandoptimizationofexperimentalcolitisinductioninratsusing246trinitrobenzenesulfonicacid
AT andalibs validationandoptimizationofexperimentalcolitisinductioninratsusing246trinitrobenzenesulfonicacid
AT rabbanim validationandoptimizationofexperimentalcolitisinductioninratsusing246trinitrobenzenesulfonicacid
AT mahzounip validationandoptimizationofexperimentalcolitisinductioninratsusing246trinitrobenzenesulfonicacid
AT afsharipourm validationandoptimizationofexperimentalcolitisinductioninratsusing246trinitrobenzenesulfonicacid
AT minaiyanm validationandoptimizationofexperimentalcolitisinductioninratsusing246trinitrobenzenesulfonicacid