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FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nuclei

BACKGROUND: The male germline in flowering plants differentiates by asymmetric division of haploid uninucleated microspores, giving rise to a vegetative cell enclosing a smaller generative cell, which eventually undergoes a second mitosis to originate two sperm cells. The vegetative cell and the spe...

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Detalles Bibliográficos
Autores principales:	Borges, Filipe, Gardner, Rui, Lopes, Telma, Calarco, Joseph P, Boavida, Leonor C, Slotkin, R Keith, Martienssen, Robert A, Becker, Jörg D
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502443/ https://www.ncbi.nlm.nih.gov/pubmed/23075219 http://dx.doi.org/10.1186/1746-4811-8-44

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