Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers

BACKGROUND: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. METHOD...

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Detalles Bibliográficos
Autores principales: Yu, Yong, Pang, Yunwei, Zhao, Haichao, Xu, Xiaoling, Wu, Zhonghong, An, Lei, Tian, Jianhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502498/
https://www.ncbi.nlm.nih.gov/pubmed/23140330
http://dx.doi.org/10.1186/2049-1891-3-35
Descripción
Sumario:BACKGROUND: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. METHODS: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. RESULTS: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. CONCLUSIONS: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.

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