Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers

BACKGROUND: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. METHOD...

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Detalles Bibliográficos
Autores principales: Yu, Yong, Pang, Yunwei, Zhao, Haichao, Xu, Xiaoling, Wu, Zhonghong, An, Lei, Tian, Jianhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502498/
https://www.ncbi.nlm.nih.gov/pubmed/23140330
http://dx.doi.org/10.1186/2049-1891-3-35
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author Yu, Yong
Pang, Yunwei
Zhao, Haichao
Xu, Xiaoling
Wu, Zhonghong
An, Lei
Tian, Jianhui
author_facet Yu, Yong
Pang, Yunwei
Zhao, Haichao
Xu, Xiaoling
Wu, Zhonghong
An, Lei
Tian, Jianhui
author_sort Yu, Yong
collection PubMed
description BACKGROUND: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. METHODS: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. RESULTS: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. CONCLUSIONS: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.
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spelling pubmed-35024982012-11-27 Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers Yu, Yong Pang, Yunwei Zhao, Haichao Xu, Xiaoling Wu, Zhonghong An, Lei Tian, Jianhui J Anim Sci Biotechnol Research BACKGROUND: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. METHODS: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. RESULTS: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. CONCLUSIONS: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers. BioMed Central 2012-11-09 /pmc/articles/PMC3502498/ /pubmed/23140330 http://dx.doi.org/10.1186/2049-1891-3-35 Text en Copyright ©2012 Yu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Yu, Yong
Pang, Yunwei
Zhao, Haichao
Xu, Xiaoling
Wu, Zhonghong
An, Lei
Tian, Jianhui
Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers
title Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers
title_full Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers
title_fullStr Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers
title_full_unstemmed Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers
title_short Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers
title_sort association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (lhcgr) with superovulation traits in chinese holstein heifers
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502498/
https://www.ncbi.nlm.nih.gov/pubmed/23140330
http://dx.doi.org/10.1186/2049-1891-3-35
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