Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus

The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarc...

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Autores principales: Lobasso, Simona, Lopalco, Patrizia, Angelini, Roberto, Vitale, Rita, Huber, Harald, Müller, Volker, Corcelli, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502756/
https://www.ncbi.nlm.nih.gov/pubmed/23193375
http://dx.doi.org/10.1155/2012/957852
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author Lobasso, Simona
Lopalco, Patrizia
Angelini, Roberto
Vitale, Rita
Huber, Harald
Müller, Volker
Corcelli, Angela
author_facet Lobasso, Simona
Lopalco, Patrizia
Angelini, Roberto
Vitale, Rita
Huber, Harald
Müller, Volker
Corcelli, Angela
author_sort Lobasso, Simona
collection PubMed
description The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, the N-acetylglucosamine-diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea.
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spelling pubmed-35027562012-11-28 Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus Lobasso, Simona Lopalco, Patrizia Angelini, Roberto Vitale, Rita Huber, Harald Müller, Volker Corcelli, Angela Archaea Research Article The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, the N-acetylglucosamine-diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea. Hindawi Publishing Corporation 2012-11-08 /pmc/articles/PMC3502756/ /pubmed/23193375 http://dx.doi.org/10.1155/2012/957852 Text en Copyright © 2012 Simona Lobasso et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lobasso, Simona
Lopalco, Patrizia
Angelini, Roberto
Vitale, Rita
Huber, Harald
Müller, Volker
Corcelli, Angela
Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus
title Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus
title_full Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus
title_fullStr Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus
title_full_unstemmed Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus
title_short Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus
title_sort coupled tlc and maldi-tof/ms analyses of the lipid extract of the hyperthermophilic archaeon pyrococcus furiosus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502756/
https://www.ncbi.nlm.nih.gov/pubmed/23193375
http://dx.doi.org/10.1155/2012/957852
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