High-throughput karyotyping of human pluripotent stem cells

Genomic integrity of human pluripotent stem cell (hPSC) lines requires routine monitoring. We report here that novel karyotyping assay, utilizing bead-bound bacterial artificial chromosome probes, provides a fast and easy tool for detection of chromosomal abnormalities in hPSC lines. The analysis ca...

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Detalles Bibliográficos
Autores principales: Lund, Riikka J., Nikula, Tuomas, Rahkonen, Nelly, Närvä, Elisa, Baker, Duncan, Harrison, Neil, Andrews, Peter, Otonkoski, Timo, Lahesmaa, Riitta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502865/
https://www.ncbi.nlm.nih.gov/pubmed/22877823
http://dx.doi.org/10.1016/j.scr.2012.06.008
Descripción
Sumario:Genomic integrity of human pluripotent stem cell (hPSC) lines requires routine monitoring. We report here that novel karyotyping assay, utilizing bead-bound bacterial artificial chromosome probes, provides a fast and easy tool for detection of chromosomal abnormalities in hPSC lines. The analysis can be performed from low amounts of DNA isolated from whole cell pools with simple data analysis interface. The method enables routine screening of stem cell lines in a cost-efficient high-throughput manner.

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