High-throughput karyotyping of human pluripotent stem cells

Genomic integrity of human pluripotent stem cell (hPSC) lines requires routine monitoring. We report here that novel karyotyping assay, utilizing bead-bound bacterial artificial chromosome probes, provides a fast and easy tool for detection of chromosomal abnormalities in hPSC lines. The analysis ca...

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Detalles Bibliográficos
Autores principales: Lund, Riikka J., Nikula, Tuomas, Rahkonen, Nelly, Närvä, Elisa, Baker, Duncan, Harrison, Neil, Andrews, Peter, Otonkoski, Timo, Lahesmaa, Riitta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502865/
https://www.ncbi.nlm.nih.gov/pubmed/22877823
http://dx.doi.org/10.1016/j.scr.2012.06.008
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author Lund, Riikka J.
Nikula, Tuomas
Rahkonen, Nelly
Närvä, Elisa
Baker, Duncan
Harrison, Neil
Andrews, Peter
Otonkoski, Timo
Lahesmaa, Riitta
author_facet Lund, Riikka J.
Nikula, Tuomas
Rahkonen, Nelly
Närvä, Elisa
Baker, Duncan
Harrison, Neil
Andrews, Peter
Otonkoski, Timo
Lahesmaa, Riitta
author_sort Lund, Riikka J.
collection PubMed
description Genomic integrity of human pluripotent stem cell (hPSC) lines requires routine monitoring. We report here that novel karyotyping assay, utilizing bead-bound bacterial artificial chromosome probes, provides a fast and easy tool for detection of chromosomal abnormalities in hPSC lines. The analysis can be performed from low amounts of DNA isolated from whole cell pools with simple data analysis interface. The method enables routine screening of stem cell lines in a cost-efficient high-throughput manner.
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spelling pubmed-35028652012-12-05 High-throughput karyotyping of human pluripotent stem cells Lund, Riikka J. Nikula, Tuomas Rahkonen, Nelly Närvä, Elisa Baker, Duncan Harrison, Neil Andrews, Peter Otonkoski, Timo Lahesmaa, Riitta Stem Cell Res Short Report Genomic integrity of human pluripotent stem cell (hPSC) lines requires routine monitoring. We report here that novel karyotyping assay, utilizing bead-bound bacterial artificial chromosome probes, provides a fast and easy tool for detection of chromosomal abnormalities in hPSC lines. The analysis can be performed from low amounts of DNA isolated from whole cell pools with simple data analysis interface. The method enables routine screening of stem cell lines in a cost-efficient high-throughput manner. Elsevier 2012-11 /pmc/articles/PMC3502865/ /pubmed/22877823 http://dx.doi.org/10.1016/j.scr.2012.06.008 Text en © 2012 Elsevier B.V. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Short Report
Lund, Riikka J.
Nikula, Tuomas
Rahkonen, Nelly
Närvä, Elisa
Baker, Duncan
Harrison, Neil
Andrews, Peter
Otonkoski, Timo
Lahesmaa, Riitta
High-throughput karyotyping of human pluripotent stem cells
title High-throughput karyotyping of human pluripotent stem cells
title_full High-throughput karyotyping of human pluripotent stem cells
title_fullStr High-throughput karyotyping of human pluripotent stem cells
title_full_unstemmed High-throughput karyotyping of human pluripotent stem cells
title_short High-throughput karyotyping of human pluripotent stem cells
title_sort high-throughput karyotyping of human pluripotent stem cells
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502865/
https://www.ncbi.nlm.nih.gov/pubmed/22877823
http://dx.doi.org/10.1016/j.scr.2012.06.008
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