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Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris

BACKGROUND: Lipase from Rhizopus chinensis is a versatile biocatalyst for various bioconversions and has been expressed at high-level in Pichia pastoris. However, the use of R. chinensis lipase in industrial applications is restricted by its low thermostability. Directed evolution has been proven to...

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Detalles Bibliográficos
Autores principales:	Yu, Xiao-Wei, Wang, Rui, Zhang, Meng, Xu, Yan, Xiao, Rong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503730/ https://www.ncbi.nlm.nih.gov/pubmed/22866667 http://dx.doi.org/10.1186/1475-2859-11-102

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