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A modified rat model of isolated bilateral pulmonary contusion

The aim of the present study was to create a feasible specific rat model of isolated bilateral pulmonary contusion (PC) and to evaluate the relationship between severity of hypoxemia and quantity of contusion lesions. Anesthetized rats were placed in a prone position. Injury energy ranging from 2.1...

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Autores principales: WANG, SHAOHUA, RUAN, ZHENG, ZHANG, JIE, ZHENG, JIN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503847/
https://www.ncbi.nlm.nih.gov/pubmed/23181112
http://dx.doi.org/10.3892/etm.2012.615
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author WANG, SHAOHUA
RUAN, ZHENG
ZHANG, JIE
ZHENG, JIN
author_facet WANG, SHAOHUA
RUAN, ZHENG
ZHANG, JIE
ZHENG, JIN
author_sort WANG, SHAOHUA
collection PubMed
description The aim of the present study was to create a feasible specific rat model of isolated bilateral pulmonary contusion (PC) and to evaluate the relationship between severity of hypoxemia and quantity of contusion lesions. Anesthetized rats were placed in a prone position. Injury energy ranging from 2.1 to 3.0 J was produced by a falling weight passed through a specially designed arched shield to the bilateral chest wall of rats. After injury (4 h), the contusion volume was measured using computer-generated three-dimensional reconstruction from a chest computed tomographic scan and expressed as a percentage of total lung volume. Arterial partial pressure of oxygen (PaO(2)) in blood gas analysis and contusion volume percentage were used to assess the severity of contusion. Heart and lung biopsy was used to confirm the diagnosis and rule out the existence of myocardial contusion. There were 3 cases of death and 1 case of death in the 3.0 J and the 2.4 J group, respectively. PaO(2) in the 2.7 J group was significantly lower than that in the lower energy groups (P<0.001). The percentage of pulmonary contusion in the 2.7 J group was significantly higher compared to that of the lower energy groups (P<0.001). PaO(2) was negatively correlated with contusion percentage (R(2)=0.76). Hemorrhage, edema and neutrophil infiltration were determined by lung biopsy. No evidence of myocardial contusion was documented in multiple heart biopsies. The method illustrated in this research effectively duplicates isolated bilateral pulmonary contusion in rats, the severity of which is highly correlated with the contusion size. Thus, 2.7 J can be regarded as the maximal energy for sublethal injury.
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spelling pubmed-35038472013-09-01 A modified rat model of isolated bilateral pulmonary contusion WANG, SHAOHUA RUAN, ZHENG ZHANG, JIE ZHENG, JIN Exp Ther Med Articles The aim of the present study was to create a feasible specific rat model of isolated bilateral pulmonary contusion (PC) and to evaluate the relationship between severity of hypoxemia and quantity of contusion lesions. Anesthetized rats were placed in a prone position. Injury energy ranging from 2.1 to 3.0 J was produced by a falling weight passed through a specially designed arched shield to the bilateral chest wall of rats. After injury (4 h), the contusion volume was measured using computer-generated three-dimensional reconstruction from a chest computed tomographic scan and expressed as a percentage of total lung volume. Arterial partial pressure of oxygen (PaO(2)) in blood gas analysis and contusion volume percentage were used to assess the severity of contusion. Heart and lung biopsy was used to confirm the diagnosis and rule out the existence of myocardial contusion. There were 3 cases of death and 1 case of death in the 3.0 J and the 2.4 J group, respectively. PaO(2) in the 2.7 J group was significantly lower than that in the lower energy groups (P<0.001). The percentage of pulmonary contusion in the 2.7 J group was significantly higher compared to that of the lower energy groups (P<0.001). PaO(2) was negatively correlated with contusion percentage (R(2)=0.76). Hemorrhage, edema and neutrophil infiltration were determined by lung biopsy. No evidence of myocardial contusion was documented in multiple heart biopsies. The method illustrated in this research effectively duplicates isolated bilateral pulmonary contusion in rats, the severity of which is highly correlated with the contusion size. Thus, 2.7 J can be regarded as the maximal energy for sublethal injury. D.A. Spandidos 2012-09 2012-06-18 /pmc/articles/PMC3503847/ /pubmed/23181112 http://dx.doi.org/10.3892/etm.2012.615 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
WANG, SHAOHUA
RUAN, ZHENG
ZHANG, JIE
ZHENG, JIN
A modified rat model of isolated bilateral pulmonary contusion
title A modified rat model of isolated bilateral pulmonary contusion
title_full A modified rat model of isolated bilateral pulmonary contusion
title_fullStr A modified rat model of isolated bilateral pulmonary contusion
title_full_unstemmed A modified rat model of isolated bilateral pulmonary contusion
title_short A modified rat model of isolated bilateral pulmonary contusion
title_sort modified rat model of isolated bilateral pulmonary contusion
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503847/
https://www.ncbi.nlm.nih.gov/pubmed/23181112
http://dx.doi.org/10.3892/etm.2012.615
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