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Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages

The formyl peptide receptor 1 (FPR1) is mainly expressed by mammalian phagocytic leukocytes and plays a role in chemotaxis, killing of microorganisms through phagocytosis, and the generation of reactive oxygen species. A large number of ligands have been identified triggering FPR1 including formylat...

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Autores principales: Gemperle, Claudio, Schmid, Mattia, Herova, Magdalena, Marti-Jaun, Jacqueline, Wuest, Sophia J. A., Loretz, Christa, Hersberger, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503994/
https://www.ncbi.nlm.nih.gov/pubmed/23185575
http://dx.doi.org/10.1371/journal.pone.0050195
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author Gemperle, Claudio
Schmid, Mattia
Herova, Magdalena
Marti-Jaun, Jacqueline
Wuest, Sophia J. A.
Loretz, Christa
Hersberger, Martin
author_facet Gemperle, Claudio
Schmid, Mattia
Herova, Magdalena
Marti-Jaun, Jacqueline
Wuest, Sophia J. A.
Loretz, Christa
Hersberger, Martin
author_sort Gemperle, Claudio
collection PubMed
description The formyl peptide receptor 1 (FPR1) is mainly expressed by mammalian phagocytic leukocytes and plays a role in chemotaxis, killing of microorganisms through phagocytosis, and the generation of reactive oxygen species. A large number of ligands have been identified triggering FPR1 including formylated and non-formylated peptides of microbial and endogenous origin. While the expression of FPR1 in neutrophils has been investigated intensively, knowledge on the regulation of FPR1 expression in polarized macrophages is lacking. In this study we show that primary human neutrophils, monocytes and resting macrophages do express the receptor on their cell surface. Polarization of macrophages with IFNγ, LPS and with the TLR8 ligand 3M-002 further increases FPR1 mRNA levels but does not consistently increase protein expression or chemotaxis towards the FPR1 ligand fMLF. In contrast, polarization of primary human macrophages with IL-4 and IL-13 leading to the alternative activated macrophages, reduces FPR1 cell surface expression and abolishes chemotaxis towards fMLF. These results show that M2 macrophages will not react to triggering of FPR1, limiting the role for FPR1 to chemotaxis and superoxide production of resting and pro-inflammatory M1 macrophages.
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spelling pubmed-35039942012-11-26 Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages Gemperle, Claudio Schmid, Mattia Herova, Magdalena Marti-Jaun, Jacqueline Wuest, Sophia J. A. Loretz, Christa Hersberger, Martin PLoS One Research Article The formyl peptide receptor 1 (FPR1) is mainly expressed by mammalian phagocytic leukocytes and plays a role in chemotaxis, killing of microorganisms through phagocytosis, and the generation of reactive oxygen species. A large number of ligands have been identified triggering FPR1 including formylated and non-formylated peptides of microbial and endogenous origin. While the expression of FPR1 in neutrophils has been investigated intensively, knowledge on the regulation of FPR1 expression in polarized macrophages is lacking. In this study we show that primary human neutrophils, monocytes and resting macrophages do express the receptor on their cell surface. Polarization of macrophages with IFNγ, LPS and with the TLR8 ligand 3M-002 further increases FPR1 mRNA levels but does not consistently increase protein expression or chemotaxis towards the FPR1 ligand fMLF. In contrast, polarization of primary human macrophages with IL-4 and IL-13 leading to the alternative activated macrophages, reduces FPR1 cell surface expression and abolishes chemotaxis towards fMLF. These results show that M2 macrophages will not react to triggering of FPR1, limiting the role for FPR1 to chemotaxis and superoxide production of resting and pro-inflammatory M1 macrophages. Public Library of Science 2012-11-21 /pmc/articles/PMC3503994/ /pubmed/23185575 http://dx.doi.org/10.1371/journal.pone.0050195 Text en © 2012 Gemperle et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gemperle, Claudio
Schmid, Mattia
Herova, Magdalena
Marti-Jaun, Jacqueline
Wuest, Sophia J. A.
Loretz, Christa
Hersberger, Martin
Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
title Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
title_full Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
title_fullStr Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
title_full_unstemmed Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
title_short Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
title_sort regulation of the formyl peptide receptor 1 (fpr1) gene in primary human macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503994/
https://www.ncbi.nlm.nih.gov/pubmed/23185575
http://dx.doi.org/10.1371/journal.pone.0050195
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