Evaluating Methods for Isolating Total RNA and Predicting the Success of Sequencing Phylogenetically Diverse Plant Transcriptomes

Next-generation sequencing plays a central role in the characterization and quantification of transcriptomes. Although numerous metrics are purported to quantify the quality of RNA, there have been no large-scale empirical evaluations of the major determinants of sequencing success. We used a combin...

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Detalles Bibliográficos
Autores principales: Johnson, Marc T. J., Carpenter, Eric J., Tian, Zhijian, Bruskiewich, Richard, Burris, Jason N., Carrigan, Charlotte T., Chase, Mark W., Clarke, Neil D., Covshoff, Sarah, dePamphilis, Claude W., Edger, Patrick P., Goh, Falicia, Graham, Sean, Greiner, Stephan, Hibberd, Julian M., Jordon-Thaden, Ingrid, Kutchan, Toni M., Leebens-Mack, James, Melkonian, Michael, Miles, Nicholas, Myburg, Henrietta, Patterson, Jordan, Pires, J. Chris, Ralph, Paula, Rolf, Megan, Sage, Rowan F., Soltis, Douglas, Soltis, Pamela, Stevenson, Dennis, Stewart, C. Neal, Surek, Barbara, Thomsen, Christina J. M., Villarreal, Juan Carlos, Wu, Xiaolei, Zhang, Yong, Deyholos, Michael K., Wong, Gane Ka-Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3504007/
https://www.ncbi.nlm.nih.gov/pubmed/23185583
http://dx.doi.org/10.1371/journal.pone.0050226

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